A Novel Colorimetric PCR-Based Biosensor for Detection and Quantification of Hepatitis B Virus
A Novel Colorimetric PCR-Based Biosensor for Detection and Quantification of Hepatitis B Virus
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一种基于比色 PCR 的新型生物传感器，用于乙型肝炎病毒的检测和定量
DOI：
10.1007/978-1-4939-6848-0_22
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发表时间：
2017-01-01
期刊：
影响因子：
--
通讯作者：
Tang, Zhuo
中科院分区：
文献类型：
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作者：
Yang, Li;Li, Mei;Tang, Zhuo
乙型肝炎病毒（HBV）可引起攻击肝脏的病毒感染，这是一个主要的全球健康问题，使人们面临因肝硬化和肝癌死亡的高风险。乙型肝炎病毒已经感染了全球三分之一的人口，并且有3.5亿人患有慢性乙型肝炎病毒感染。因此，开发一种准确、灵敏、便捷的检测方法来诊断、监测和评估乙型肝炎的治疗反应对于公共卫生和疾病控制来说是非常必要和紧迫的。在这里，我们报告了一种基于比色聚合酶链反应（PCR）和含 DNAzyme 探针的 HBV 病毒载量定量检测新策略。采用 G-四链体结构的特殊 DNAzyme 在氯化血红素存在下表现出类似过氧化物酶的活性，以报告比色信号。该方法表现出广泛的线性范围和高灵敏度。该研究为以低成本、有效的方法实现HBV DNA特异性、准确的检测水平，帮助全世界特别是发展中国家的HBV诊断、预防和保护人类健康奠定了重要基础。
Hepatitis B virus (HBV) can cause viral infection that attacks the liver and it is a major global health problem that puts people at a high risk of death from cirrhosis of the liver and liver cancer. HBV has infected one-third of the worldwide population, and 350 million people suffer from chronic HBV infection. For these reasons, development of an accurate, sensitive, and expedient detection method for diagnosing, monitoring, and assessing therapeutic response of HBV is very necessary and urgent for public health and disease control. Here we report a new strategy for detection of viral load quantitation of HBV based on colorimetric polymerase chain reaction (PCR) with DNAzyme-containing probe. The special DNAzyme adopting a G-quadruplex structure exhibited peroxidase-like activity in the presence of hemin to report colorimetric signal. This method has shown a broad range of linearity and high sensitivity. This study builds an important foundation to achieve the specific and accurate detection level of HBV DNA with a low-cost and effective method in helping diagnosing, preventing and protecting human health form HBV all over the world, and especially in developing countries.