Monitoring of intense neuromuscular blockade in a pig model
Monitoring of intense neuromuscular blockade in a pig model
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DOI：
10.1007/s10877-013-9539-1
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发表时间：
2014-08-01
影响因子：
2.2
通讯作者：
Gatke, Mona R.
中科院分区：
文献类型：
--
作者：
Madsen, Matias V.;Donatsky, Anders M.;Gatke, Mona R.
在该猪模型中，对通过强直后计数 (PTC) 测量的强烈神经肌肉阻滞 (NMB) 进行了监测、逆转和验证。在交叉评估者盲法设计中，六头猪被随机分配到不使用 NMB，然后使用强 NMB，或使用强 NMB，然后不使用 NMB。神经肌肉测量采用肌加速描记法[四人组 (TOF) Watch SX] 进行。在所有猪中，用罗库溴铵 3 mg/kg 建立的 TOF 神经刺激反应稳定且强烈的 NMB (PTC 0-1)。为了实现逆转，猪接受了 20-35 mg/kg 的舒更葡糖，注射后 2.15 分钟内 TOF 比率恢复到 0.90 以上。我们建立了一个猪模型，用于通过表面刺激电极和肌加速描记术监测强烈的 NMB。我们通过抽吸测试和表面肌电图验证了 PTC 0-1 处隔膜和腹部肌肉的完全松弛。该猪模型适用于监测强烈 NMB 的实验性腹部手术研究，以及需要松弛膈肌和腹部肌肉的情况。
Intense neuromuscular blockade (NMB) measured by post tetanic count (PTC) was monitored, reversed and verified in this pig model. In a cross-over assessor blinded design six pigs were randomized to either no NMB followed by intense NMB, or intense NMB followed by no NMB. Neuromuscular measurements were performed with acceleromyography [train-of-four (TOF) Watch SX]. In all pigs, the response to TOF nerve stimulation was stable and intense NMB (PTC 0-1) was established with rocuronium 3 mg/kg. For reversal, the pigs received sugammadex 20-35 mg/kg and returned to TOF-ratio above 0.90 within 2.15 min after injection. We established a pig model for monitoring intense NMB with surface stimulation electrodes and acceleromyography. We verified total relaxation of the diaphragm and the abdominal muscles at the PTC 0-1 by suction test and with surface electromyography. This pig model is suitable for studies with experimental abdominal surgery with monitoring of intense NMB, and where relaxation of the diaphragm and the abdominal muscles are required.