Determination of hydroxysafflor yellow A in biological fluids of patients with traumatic brain injury by UPLC-ESI-MS/MS after injection of Xuebijing
Determination of hydroxysafflor yellow A in biological fluids of patients with traumatic brain injury by UPLC-ESI-MS/MS after injection of Xuebijing
复制标题
UPLC-ESI-MS/MS法测定脑外伤患者注射血必净后生物体液中羟基红花黄色素A的含量
DOI：
10.1002/bmc.3124
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发表时间：
2014-08-01
影响因子：
1.8
通讯作者：
Liu, Jinfang
中科院分区：
文献类型：
--
作者：
Guo, Yong;Wang, Yang;Liu, Jinfang
建立了一种简便、特异、快速、重复性好的超高效液相色谱-电喷雾串联质谱法测定脑外伤患者静脉注射血必净后生物体液（血浆、尿液和脑脊液）中羟色胺黄A（HSYA）的方法。采用液-液萃取法，采用Acquity UPLC（TM）BEH C-18色谱柱，以甲醇和0.1%甲酸组成的移动的流动相梯度洗脱，流速为0.3 mL/min，电喷雾三重四极杆串联质谱仪检测。随后的质量转变为m/z 611.0 -> 491。保留时间小于3.0 min。脑脊液、血浆和尿液的浓度范围为2 - 6125 ng/mL，校准曲线呈线性。日内和日间精密度为
A simple, novel, specific, rapid and reproducible ultra-performance liquid chromatography electrospray ionization tandem mass spectrometry method has been developed and validated for the determination of hydroxysafflor yellow A (HSYA) in biological fluids (plasma, urine and cerebrospinal fluid) of patients with traumatic brain injury after intravenous injection of Xuebijing (XBJ). Liquid-liquid extraction was performed, and separation was carried out on an Acquity UPLC (TM) BEH C-18 column, with gradient elution using a mobile phase composed of methanol and 0.1% formic acid at a flow rate of 0.3 mL/min. A triple quadrupole tandem mass spectrometer with electrospray ionization was used for the detection of HSYA. The mass transition followed was m/z 611.0 -> 491. The retention time was less than 3.0 min. The calibration curve was linear in the concentration range from 2 to 6125 ng/mL for cerebrospinal fluid, plasma and urine. The intra-and inter-day precisions were