IL-4 dependent resistance to the tapeworm Mesocestoides corti (Cestoda) in mice
IL-4 dependent resistance to the tapeworm Mesocestoides corti (Cestoda) in mice
复制标题
DOI：
10.1111/j.0141-9838.2004.00666.x
复制
发表时间：
2003-11-01
影响因子：
2.2
通讯作者：
Taylor, MJ
中科院分区：
文献类型：
--
作者：
Rawat, J;Dixon, JB;Taylor, MJ
C57 BL/6小鼠的三周中囊线虫感染显示在具有IL-4基因的靶向敲除的动物中寄生虫计数显著升高。相比之下，抗体中和IL-5和抑制嗜酸性粒细胞对寄生虫数量没有影响。在SV/129小鼠中，干扰素γ受体和诱导型一氧化氮合酶基因敲除对寄生虫计数没有显著影响。在IL-4（-/-）小鼠中，优势IgG 1抗体应答显著降低，同时IgG 2a/B应答增加，IgM和IgE应答部分降低两倍。我们的结论是小鼠对M. corti依赖于IL-4，但不依赖于IL-5和嗜酸性粒细胞。这为IL-4介导的小鼠对组织寄居的扁形动物感染的免疫提供了第一个直接证据。
Three-week Mesocestoides corti infections of C57BL/6 mice showed significantly raised parasite counts in animals with a targeted knockout of the IL-4 gene. By contrast, antibody neutralization of IL-5 and inhibition of eosinophilia had no effect on parasite numbers. In SV/129 mice, knockout of the interferon gamma receptor and inducible nitric oxide synthase genes had no significant effect on parasite counts. In IL-4(-/-)mice the dominant IgG1 antibody response was dramatically reduced, with a concomitant increase in IgG2a/b responses and a partial twofold reduction in IgM and IgE responses. We conclude that murine resistance to M. corti is dependent on IL-4, but occurs independently of IL-5 and eosinophils. This provides the first direct evidence for IL-4 mediated immunity of mice to infection with a tissue-dwelling platyhelminth.