A microneedle patch containing measles vaccine is immunogenic in non-human primates.
A microneedle patch containing measles vaccine is immunogenic in non-human primates.
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DOI：
10.1016/j.vaccine.2015.02.074
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发表时间：
2015-09-08
期刊：
影响因子：
5.5
通讯作者：
Prausnitz MR
中科院分区：
文献类型：
--
作者：
Edens C;Collins ML;Goodson JL;Rota PA;Prausnitz MR
要消除麻疹，需要非常高的疫苗接种覆盖率，但实现高覆盖率可能会受到皮下注射相关后勤挑战的限制。为了简化疫苗递送的物流，配制含有微米级聚合物针的贴剂以封装标准剂量的麻疹疫苗（1000 TCID50），并将微针贴剂的免疫原性与恒河猴皮下注射进行比较。微针贴剂给药时未用稀释剂复溶，10分钟内在皮肤中溶解，仅引起轻度、一过性皮肤红斑。两组恒河猴产生的麻疹中和抗体反应与保护一致，中和抗体滴度相当。此外，微针贴片在高温下储存后保持可接受的效力水平，表明与标准冻干疫苗相比热稳定性改善。总之，麻疹微针贴片疫苗在非人灵长类动物中具有免疫原性，这种方法提供了一种有希望的递送方法，可以帮助提高疫苗接种覆盖率。
Very high vaccination coverage is required to eliminate measles, but achieving high coverage can be constrained by the logistical challenges associated with subcutaneous injection. To simplify logistics of vaccine delivery, a patch containing micron-scale polymeric needles was formulated to encapsulate the standard dose of measles vaccine (1000 TCID50) and the immunogenicity of the microneedle patch was compared with subcutaneous injection in rhesus macaques. The microneedle patch was administered without reconstitution with diluent, dissolved in skin within 10 minutes, and caused only mild, transient skin erythema. Both groups of rhesus macaques generated neutralizing antibody responses to measles that were consistent with protection and the neutralizing antibody titers were equivalent. In addition, the microneedle patches maintained an acceptable level of potency after storage at elevated temperature suggesting improved thermostability compared to standard lyophilized vaccine. In conclusion, a measles microneedle patch vaccine was immunogenic in non-human primates, and this approach offers a promising delivery method that could help increase vaccination coverage.