In Vivo Visualization of Microglia Using Tomato Lectin
In Vivo Visualization of Microglia Using Tomato Lectin
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DOI：
10.1007/978-1-4939-9658-2_12
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发表时间：
2019-01-01
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影响因子：
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通讯作者：
Garaschuk, Olga
中科院分区：
文献类型：
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作者：
Brawek, Bianca;del Moral, Maria Olmedillas;Garaschuk, Olga
植物凝集素与碳水化合物结合，碳水化合物存在于不同的免疫细胞和内皮细胞（包括小胶质细胞）的表面。使用市售的凝集素与不同荧光团的缀合物允许一步检测和可视化的小胶质细胞在体内。在这里，我们描述了一个协议，使使用一个特定的植物凝集素分离番茄。番茄凝集素能够在体内高质量地标记小胶质细胞，并且适用于任何年龄的实验动物的任何小鼠品系，而不需要与时间和资源消耗程序相关的遗传标记。
Plant lectins bind to carbohydrates, which are found on the surface of different immune and endothelial cells including microglia. Using commercially available conjugates of lectins with different fluorophores allows one-step detection and visualization of microglia in vivo. Here, we describe a protocol enabling the use of a specific plant lectin isolated from Lycopersicon esculentum. Tomato lectin enables high-quality labeling of microglial cells in vivo and is applicable in any mouse strain at any age of the experimental animal without the need of genetic labeling, which is associated with time- and resource-consuming procedures.