Studies on infiltrating host cells harvested from acutely rejecting rat cardiac allografts.
Studies on infiltrating host cells harvested from acutely rejecting rat cardiac allografts.
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对从急性排斥大鼠同种异体心脏移植物中收获的浸润宿主细胞的研究。
DOI：
10.5555/uri:pii:0039606076902907
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发表时间：
1976
期刊：
影响因子：
3.8
通讯作者：
C. Carpenter
中科院分区：
文献类型：
--
作者：
N. Tilney;T. Strom;S. Macpherson;C. Carpenter
虽然器官移植排斥反应的细胞事件已在组织学上得到充分描述，但关于移植物破坏的实际机制的信息很少。可用的概念主要基于体外进行的测定。本研究描述了一种模型，旨在将这些体外信息与急性排斥器官移植物体内发生的事件相关联。用两种方法从大鼠异位心脏移植物中获得浸润的宿主细胞。已经注意到各种细胞类别，并将其累积速率与系列组织学观察结果进行比较。细胞亚群已通过表面标志物鉴定，针对供体同种异体抗原的特异性细胞毒性已被确定为T淋巴细胞功能。这些技术可能有助于揭示移植排斥的复杂性。
Although the cellular events of rejection of organ allografts have been fully described histologically, little information exists regarding actual mechanisms of graft destruction. Available concepts are based primarily upon assays performed in vitro. This study describes a model designed to correlate such in vitro information with events occurring in vivo within acutely rejecting organ allografts. Infiltrating host cells had been harvested by two techniques from heterotopic cardiac allografts in rats. The various cell classes have been noted and their differential rates of accumulation compared to serial histological observations. Subpopulations of cells have been identified by surface markers, and specific cytotoxicity against donor alloantigen has been determined as a T lymphocyte function. These techniques may be of aid in unraveling the complexities of graft rejection.