Frequency and functional activity of Th17, Tc17 and other T-cell subsets in Systemic Lupus Erythematosus
Frequency and functional activity of Th17, Tc17 and other T-cell subsets in Systemic Lupus Erythematosus
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DOI：
10.1016/j.cellimm.2010.05.004
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发表时间：
2010-01-01
影响因子：
4.3
通讯作者：
Paiva, Artur
中科院分区：
文献类型：
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作者：
Henriques, Ana;Ines, Luis;Paiva, Artur
为了比较外周血（PB）Th(c)17、Th(c)1和调节性T细胞（Treg）的频率和功能活性以及2型细胞因子mRNA的量，我们招募了处于活动期（n = 15）和非活动期（n = 19）的系统性红斑狼疮（SLE）患者以及年龄和性别匹配的健康对照者（n = 15）。通过流式细胞术对Th(c)17、Th(c)1和Treg细胞进行研究，通过实时聚合酶链反应（PCR）对细胞因子mRNA进行研究。与正常对照（NC）相比，SLE患者的Th(c)17细胞比例增加，但每个细胞的白细胞介素 - 17（IL - 17）量较低，并且Treg细胞的频率降低，但转化生长因子 - β（TGF - β）和FoxP3 mRNA的产生增加。与非活动期SLE相比，活动期SLE的Th(c)1细胞频率显著降低，2型细胞因子mRNA的产生增加，并且Th(c)17细胞具有独特的功能特征。我们的研究结果表明，SLE中T细胞亚群存在功能失衡，这可能有助于炎症过程和疾病发病机制。（C）2010爱思唯尔公司。保留所有权利。
To compare frequency and functional activity of peripheral blood (PB) Th(c)17, Th(c)1 and Treg cells and the amount of type 2 cytokines mRNA we recruited SLE patients in active (n = 15) and inactive disease (n = 19) and healthy age- and gender-matched controls (n = 15). The study of Th(c)17, Th(c)1 and Treg cells was done by flow cytometry and cytokine mRNA by real-time PCR. Compared to NC, SLE patients present an increased proportion of Th(c)17 cells, but with lower amounts of IL-17 per cell and also a decreased frequency of Treg, but with increased production of TGF-beta and FoxP3 mRNA. In active compared to inactive SLE, there is a marked decreased in frequency of Th(c)1 cells, an increased production of type 2 cytokines mRNA and a distinct functional profile of Th(c)17 cells. Our findings suggest a functional disequilibrium of T-cell subsets in SLE which may contribute to the inflammatory process and disease pathogenesis. (C) 2010 Elsevier Inc. All rights reserved.