454 Pyrosequencing analyses of forest soils reveal an unexpectedly high fungal diversity
454 Pyrosequencing analyses of forest soils reveal an unexpectedly high fungal diversity
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DOI：
10.1111/j.1469-8137.2009.03003.x
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发表时间：
2009-01-01
期刊：
影响因子：
9.4
通讯作者：
Martin, F.
中科院分区：
文献类型：
--
作者：
Buee, M.;Reich, M.;Martin, F.
土壤真菌在森林生态和生物地球化学过程中起着重要作用。然而，对不同真菌群落的结构和丰富度以及功能生态群（病原体、物种和共生体）的分布知之甚少。在这里，我们使用标签编码的454焦磷酸测序对核糖体内转录间隔-1 （ITS-1）进行了评估，评估了6种不同森林土壤中的真菌多样性。从所有样品中获得不少于166 350个ITS读数。在每个森林土壤样本（4 g）中，大约恢复了3万个reads，对应于大约1000个分子操作分类单位。大部分操作分类单位（81%）属于Dikarya亚界（子囊菌门和担子菌门）。丰富度、丰度和分类分析表明，真菌属真菌优势纲。所分析的ITS-1序列（73%）仅对应26个分类群。最丰富的操作分类单元与Ceratobasidium sp.、Cryptococcus podzolicus、Lactarius sp.和Scleroderma sp.序列相似性最高，验证了高通量454测序技术在土壤真菌多样性调查中的有效性。然而，大量未识别的序列需要精心设计的序列数据库。对土壤样品进行焦磷酸测序将加速森林生态系统真菌群落时空动态的研究。
P>Soil fungi play a major role in ecological and biogeochemical processes in forests. Little is known, however, about the structure and richness of different fungal communities and the distribution of functional ecological groups (pathogens, saprobes and symbionts).Here, we assessed the fungal diversity in six different forest soils using tag-encoded 454 pyrosequencing of the nuclear ribosomal internal transcribed spacer-1 (ITS-1). No less than 166 350 ITS reads were obtained from all samples. In each forest soil sample (4 g), approximately 30 000 reads were recovered, corresponding to around 1000 molecular operational taxonomic units.Most operational taxonomic units (81%) belonged to the Dikarya subkingdom (Ascomycota and Basidiomycota). Richness, abundance and taxonomic analyses identified the Agaricomycetes as the dominant fungal class. The ITS-1 sequences (73%) analysed corresponded to only 26 taxa. The most abundant operational taxonomic units showed the highest sequence similarity to Ceratobasidium sp., Cryptococcus podzolicus, Lactarius sp. and Scleroderma sp.This study validates the effectiveness of high-throughput 454 sequencing technology for the survey of soil fungal diversity. The large proportion of unidentified sequences, however, calls for curated sequence databases. The use of pyrosequencing on soil samples will accelerate the study of the spatiotemporal dynamics of fungal communities in forest ecosystems.