Activity-based assessment of an engineered hyperthermophilic protein as a capture agent in paper-based diagnostic tests.

Activity-based assessment of an engineered hyperthermophilic protein as a capture agent in paper-based diagnostic tests.
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DOI:
10.1039/c6me00032k
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发表时间:
2016-12-01
影响因子:
3.6
通讯作者:
Sikes HD
Sikes HD
中科院分区:
工程技术3区
文献类型:
--
作者:
Miller EA;Traxlmayr MW;Shen J;Sikes HD

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Antibodies have traditionally served as the affinity reagents of choice in point-of-care diagnostic biosensors. However, this class of proteins is not ideally suited for this use, being poorly characterized and prone to thermal denaturation. Here, we present an activity-based assessment of an alternative engineered binding protein in a cellulose-based assay.