Arabidopsis Histone Methyltransferase SUVH5 Is a Positive Regulator of Light-Mediated Seed Germination
Arabidopsis Histone Methyltransferase SUVH5 Is a Positive Regulator of Light-Mediated Seed Germination
复制标题
拟南芥组蛋白甲基转移酶 SUVH5 是光介导种子萌发的正调节因子
DOI：
10.3389/fpls.2019.00841
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发表时间：
2019-06-27
影响因子：
5.6
通讯作者：
Liu, Xuncheng
中科院分区：
文献类型：
--
作者：
Gu, Dachuan;Ji, Rujun;Liu, Xuncheng
植物生命周期从种子萌发开始，受各种环境因素和内源激素的调节。在吸胀的初始阶段，光主要通过光敏色素 B (PHYB) 促进种子萌发，这涉及全基因组的光响应转录变化。最近的研究表明多种表观遗传因素参与种子萌发的控制。然而，关于组蛋白甲基转移酶在光介导的种子萌发过程中的作用的研究很少。在这里，我们确定了 SUVH5（一种组蛋白 H3 赖氨酸 9 甲基转移酶）作为拟南芥光介导种子萌发的正调节因子。 SUVH5 功能丧失会导致 PHYB 依赖性种子发芽率降低。 RNA测序分析显示，SUVH5调节吸水种子中24.6%的光响应转录组，主要与激素信号通路和发育过程有关。此外，SUVH5 通过吸收种子中组蛋白 H3 赖氨酸 9 (H3K9me2) 的二甲基化来抑制 ABA 生物合成和信号转导相关基因以及发芽延迟 (DOG) 基因家族的转录。综上所述，我们的研究结果表明，SUVH5 是拟南芥光介导种子萌发的新型正调节因子。
Plant lifecycle starts from seed germination, which is regulated by various environmental cues and endogenous hormones. Light promotes seed germination mainly by phytochrome B (PHYB) during the initial phase of imbibition, which involves genome-wide light-responsive transcription changes. Recent studies indicated an involvement of multiple epigenetic factors in the control of seed germination. However, few studies have been reported about the role of a histone methyltransferase in light-mediated seed germination process. Here, we identified SUVH5, a histone H3 lysine 9 methyltransferase, as a positive regulator in light-mediated seed germination in Arabidopsis. Loss of function of SUVH5 leads to decreased PHYB-dependent seed germination. RNA-sequencing analysis displayed that SUVH5 regulates 24.6% of light-responsive transcriptome in imbibed seeds, which mainly related to hormonal signaling pathways and developmental processes. Furthermore, SUVH5 represses the transcription of ABA biosynthesis and signal transduction-related genes, as well as a family of DELAY OF GERMINATION (DOG) genes via dimethylation of histone H3 at lysine 9 (H3K9me2) in imbibed seeds. Taken together, our findings revealed that SUVH5 is a novel positive regulator of light-mediated seed germination in Arabidopsis.