AN 11-BASE-PAIR SEQUENCE DETERMINES THE SPECIFICITY OF DNA UPTAKE IN HEMOPHILUS TRANSFORMATION
AN 11-BASE-PAIR SEQUENCE DETERMINES THE SPECIFICITY OF DNA UPTAKE IN HEMOPHILUS TRANSFORMATION
复制标题
11 个碱基对序列决定了嗜血杆菌转化过程中 DNA 吸收的特异性
DOI：
10.1016/0378-1119(80)90071-2
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发表时间：
1980-01-01
期刊：
影响因子：
3.5
通讯作者：
SMITH, HO
中科院分区：
文献类型：
--
作者：
DANNER, DB;DEICH, RA;SMITH, HO
只有某些DNA片段才能被感受态嗜血杆菌细胞有效吸收;这意味着有效吸收需要进入的DNA上存在特定的核苷酸序列。为了确定该摄取位点的结构，克隆了H.分离保留被细胞摄取能力的副流感病毒DNA。这些片段具有共同的11个碱基对的序列5“-AAGTGCGGTCA-3”，并且该序列中某些磷酰基的乙基化导致片段摄取的显著降低。这是摄取位点的序列。
Only certain DNA fragments are taken up efficiently by competent Haemophilus cells; this implies that efficient uptake requires the presence of a specific nucleotide sequence on the incoming DNA. To determine the structure of this uptake site, 4 small fragments of cloned H. parainfluenzae DNA were isolated which retain the ability to be taken up by cells. These fragments have a sequence of 11 base pairs in common, 5''-AAGTGCGGTCA-3'' and ethylation of certain phosphoryl groups in this sequence causes significant decreases in fragment uptake. This is the sequence of the uptake site.