Glucose Triggers ATP Secretion from Bacteria in a Growth-Phase-Dependent Manner
Glucose Triggers ATP Secretion from Bacteria in a Growth-Phase-Dependent Manner
复制标题
DOI：
10.1128/aem.03871-12
复制
发表时间：
2013-04-01
影响因子：
4.4
通讯作者：
Mizunoe, Yoshimitsu
中科院分区：
文献类型：
--
作者：
Hironaka, Ippei;Iwase, Tadayuki;Mizunoe, Yoshimitsu
ATP 调节免疫细胞功能，源自肠道共生细菌的 ATP 促进肠固有层中辅助性 T 17 (Th17) 细胞的分化。我们最近报道，从小鼠和人类体内分离出的鸡肠球菌能够分泌 ATP。此后我们发现并鉴定了几种 ATP 分泌细菌。在所测试的肠球菌中，过夜培养后，肠球菌分泌的 ATP 量最多（>2 mu M/10(8) 细胞）。 E. mundtii 分泌 ATP 所必需的是葡萄糖，而不是氨基酸和维生素。对能量剥夺细胞的分析表明，糖酵解是细菌 ATP 分泌的最重要途径。此外，指数期 E. mundtii 和粪肠球菌细胞比稳定期细胞更有效地分泌 ATP。其他细菌，包括铜绿假单胞菌、大肠杆菌和金黄色葡萄球菌，也在指数期而非稳定期分泌 ATP。这些结果表明，各种肠道细菌，包括共生菌和病原体，可能在任何生长阶段分泌 ATP 并调节免疫细胞功能。
ATP modulates immune cell functions, and ATP derived from gut commensal bacteria promotes the differentiation of T helper 17 (Th17) cells in the intestinal lamina propria. We recently reported that Enterococcus gallinarum, isolated from mice and humans, secretes ATP. We have since found and characterized several ATP-secreting bacteria. Of the tested enterococci, Enterococcus mundtii secreted the greatest amount of ATP (>2 mu M/10(8) cells) after overnight culture. Glucose, not amino acids and vitamins, was essential for ATP secretion from E. mundtii. Analyses of energy-deprived cells demonstrated that glycolysis is the most important pathway for bacterial ATP secretion. Furthermore, exponential-phase E. mundtii and Enterococcus faecalis cells secrete ATP more efficiently than stationary-phase cells. Other bacteria, including Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus, also secrete ATP in exponential but not stationary phase. These results suggest that various gut bacteria, including commensals and pathogens, might secrete ATP at any growth phase and modulate immune cell function.