Development of localized surface plasmon resonance biosensors for the detection of Brettanomyces bruxellensis in wine
Development of localized surface plasmon resonance biosensors for the detection of Brettanomyces bruxellensis in wine
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DOI：
10.1016/j.snb.2015.09.099
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发表时间：
2016-02-01
影响因子：
8.4
通讯作者：
Ionescu, Rodica Elena
中科院分区：
文献类型：
--
作者：
Manzano, Marisa;Vizzini, Priya;Ionescu, Rodica Elena
入射光与小于入射光波长的贵金属纳米颗粒相互作用，产生局域表面等离子体共振。在这项工作中，利用金纳米结构表面固定5‘端硫醇修饰的DNA探针，研制了一种用于检测扰流型葡萄酒酵母Brettanmycesbrxellens的LSPR纳米生物传感器。金被蒸发以获得厚度为4 nm的金。用2亩L的DNA和不同浓度的阴性对照检测LSPR技术的特异性和敏感性。由于DNA探针结合而引起的纳米颗粒光学性质的变化反映在LSPR消光极大值(lambda(Max))的移动上。以布鲁氏芽孢杆菌为靶标微生物，以酿酒酵母为阴性对照，验证了DNA探针和方法的特异性。LSPR分光光度技术检测到0.1 ng/亩的L DNA靶标，证实了利用该系统检测食品和饮料样品中低含量病原体微生物的可能性。(C)2015爱思唯尔B.V.保留所有权利。
Incident light interacting with noble-metal nanoparticles with smaller sizes than the wavelength of the incident light induces localized surface plasmon resonance (LSPR). In this work a gold nanostructured surface was used for the immobilization of a 5' end Thiol modified DNA probe to develop a LSPR nanobiosensor for the detection of the spoiler wine yeast Brettanomyces bruxellensis. Gold was evaporated to obtain a gold thickness of 4 nm. DNA (2 mu L) from the target microorganism and the negative control at various concentrations were used to test the specificity and sensitivity of the LSPR technique. Changes in the optical properties of the nanoparticles due to DNA-probe binding are reflected in the shift of LSPR extinction maximum (lambda(max)). The results obtained using as target microorganism B. bruxellensis, and as negative control Saccharomyces cerevisiae demonstrated the specificity of both the DNA-probe and the protocol. The LSPR spectrophotometry technique detects 0.1 ng/mu L DNA target confirming the possibility to utilize this system for the detection of pathogen microorganisms present in low amount in food and beverage samples. (C) 2015 Elsevier B.V. All rights reserved.