Transcutaneous pO2 measurement during tourniquet-induced venous occlusion using dynamic phosphorescence imaging
Transcutaneous pO2 measurement during tourniquet-induced venous occlusion using dynamic phosphorescence imaging
复制标题
DOI：
10.3233/ch-2008-1134
复制
发表时间：
2008-01-01
影响因子：
2.1
通讯作者：
Prantl, L.
中科院分区：
文献类型：
--
作者：
Geis, S.;Babilas, P.;Prantl, L.
皮肤移植物要有足够的氧气供应，就需要有功能正常的微循环。静脉闭塞损害微循环，因此是愈合的主要威胁。发光寿命成像(LLI)可以无创地、二维地评估经皮氧分压(p(TC)O(2))。在目前的试验中，这一新设备被应用于静脉充血监测。8名健康志愿者将上臂上的止血带充气至40-50毫米汞柱，10分钟后释放。使用固定在聚苯乙烯基质中的铂(II)-八乙基卟啉的LLI，在袖带阻断(应用皮温40℃)之前、期间和之后每分钟测量小臂的p(TC)O(2)。为了验证，极谱Clark电极技术被应用于近距离，同时进行测量。在使用Clark电极之前(Clark：50.68+/-5.69 mm Hgvs LLI：50.89+/-4.96 mm Hg)和在静脉充血结束时(Clark：16.41+/-4.54 mm Hgvs.LLI：23.82+/-3.23 mm Hg)，p(TC)O(2)测量没有显著差异。在充血初期分别再灌流阶段，Clark电极测量的速度较快，p(TC)O(2)因该方法的耗氧量而分别降低。本实验验证了LLI在静脉血流量变化情况下定量p(TC)O(2)的适用性。平面透明传感器的使用允许非侵入性地生成表面Po(2)的二维地图，这使得该方法特别适合于皮肤移植物的监测。
A sufficient oxygen supply in skin grafts requires a functioning microcirculation. Venous occlusion impairs the microcirculation and is therefore a major threat of healing. Luminescence life time imaging (LLI) enables the non-invasive and two-dimensional assessment of the transcutaneous oxygen partial pressure (p(tc)O(2)). In the current trial this new device was applied for monitoring of venous congestion.A tourniquet on the upper arm was inflated up to 40-50 mmHg and released after 10 min in eight healthy volunteers. The p(tc)O(2) was measured at the lower arm every minute prior to, during and up to 10 min after cuff occlusion (40 degrees C applied skin temperature) using LLI of platinum(II)-octaethyl-porphyrin immobilized in a polystyrene matrix. For validation the polarographic Clark electrode technique was applied in close proximity and measurement was performed simultaneously.p(tc)O(2) measurements prior to (Clark: 50.68 +/- 5.69 mmHg vs. LLI: 50.89 +/- 4.96 mmHg) and at the end of the venous congestion (Clark: 16.41 +/- 4.54 mmHg vs. LLI: 23.82 +/- 3.23 mmHg) did not differ significantly using the Clark electrode vs. LLI. At the initial congestion respectively reperfusion phase the Clark electrode measured faster decreases respectively increase of p(tc)O(2) due to oxygen consumption of this method.This experimental trial demonstrates the applicability of LLI to quantify the p(tc)O(2) under changing venous blood flow. The use of planar transparent sensors allows the non-invasive generation of two-dimensional maps of surface pO(2) what makes this method particular suitable for monitoring of skin grafts.