Role of thioproline on seed germination: Interaction ROS-ABA and effects on antioxidative metabolism
Role of thioproline on seed germination: Interaction ROS-ABA and effects on antioxidative metabolism
复制标题
DOI：
10.1016/j.plaphy.2011.12.002
复制
发表时间：
2012-10-01
影响因子：
6.5
通讯作者：
Diaz-Vivancos, Pedro
中科院分区：
文献类型：
--
作者：
Barba-Espin, Gregorio;Nicolas, Eduardo;Diaz-Vivancos, Pedro
本文研究了不同浓度的硫代脯氨酸(TP)对豌豆种子的吸胀作用对萌发率和幼苗早期生长的影响。本研究还分析了茶多酚与过氧化氢(H_2O_2)处理之间的相互作用，以检验在萌发和生长过程中是否发生了协同作用。尽管茶多酚对豌豆种子的吸胀没有显著提高发芽率，但TP和/或H_2O_2预处理促进了幼苗生长。这种对幼苗生长的促进作用被脱落酸(ABA)的添加所抑制。豌豆种子在ABA存在下的吸胀也降低了对照和TP处理的豌豆幼苗的内源H_2O_2含量。在没有ABA的情况下，TP和/或H_2O_2处理内源H_2O_2含量的增加可能与这些活性的降低有关。最后，对豌豆幼苗的激素谱进行了研究。结果表明，H_2O_2和TP+H_2O_2对幼苗生长的促进作用与其对ABA的抑制作用相关。内源H_2O_2含量与植物生长之间存在一定的关系，从而加强了活性氧自由基(ROS)与植物激素在种子萌发信号和幼苗早期发育过程中的相互作用。(C)2012年爱思唯尔·马森公司。版权所有。
In this work we investigate the effect of the imbibition of pea seeds with different thioproline (TP) concentrations on the germination percentage and the early growth of the seedlings. The interaction between TP and hydrogen peroxide (H2O2) treatments is also analysed in order to test if any synergy in germination and growth occurs.Although the imbibition of pea seeds in the presence of TP did not significantly improve the germination percentage, TP and/or H2O2 pre-treatments increased seedlings growth. This increase in seedling growth was reduced by abscisic acid (ABA) addition. Imbibition of pea seeds in the presence of ABA also reduced the endogenous H2O2 contents of pea seedlings in control and TP-treated seeds.The incubation of pea seeds with TP and/or H2O2 in presence or absence of ABA decreased the activity of H2O2-scavenging enzymes. The increase of the endogenous H2O2 contents observed in TP and/or H2O2 treatments in absence of ABA could be correlated with the decrease in these activities. Finally, the hormone profile of pea seedlings was investigated. The results show that the increase in seedling growth is correlated with a decrease in ABA in samples pre-treated with H2O2 and TP + H2O2. Nevertheless, no significant differences in endogenous ABA concentration were observed with the TP pre-treatment.This paper suggests a relationship between endogenous H2O2 contents and plant growth, so reinforcing the intricate crosstalk between reactive oxygen species (ROS) and plant hormones in seed germination signalling and early seedling development. (c) 2012 Elsevier Masson SAS. All rights reserved.