Expression Quantitative Trait Loci Analysis of Two Genes Encoding Rubisco Activase in Soybean
Expression Quantitative Trait Loci Analysis of Two Genes Encoding Rubisco Activase in Soybean
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大豆Rubisco激活酶两个基因的表达数量性状位点分析
DOI：
10.1104/pp.109.148312
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发表时间：
2010-03-01
期刊：
影响因子：
7.4
通讯作者：
Yu, Deyue
中科院分区：
文献类型：
--
作者：
Yin, Zhitong;Meng, Fanfan;Yu, Deyue
Rubisco活化酶（RCA）是一类在体内催化Rubisco活化的酶，在光合作用中起着重要作用。然而，到目前为止，对最重要的豆类作物之一大豆（Glycine max）的RCA的分子遗传学知之甚少。在这里，我们克隆和表征了两个基因编码大豆RCA的较长的α-同种型和较短的β-同种型（分别为GmRCA α和GmRCA β）。这两个相应的cDNA在翻译区和3'非翻译区都是不同的。基因组DNA序列的分析表明，相应的mRNA是两个不同基因的转录本，而不是一个单一的选择性剪接前mRNA的产物。另外还分离出两个可能的α型RCA编码基因GmRCA 03和GmRCA 14，以及一个另外的β型RCA编码基因GmRCA 11。为了研究RCA基因在大豆中的功能和调节，我们测定了184个大豆重组自交系的GmRCA α和GmRCA β的表达水平、Rubisco初始活性、光合速率和种子产量。基因表达水平与其他3个性状的相关性表明，RCA基因可能在调节大豆光合能力和种子产量方面发挥重要作用。表达数量性状基因座定位揭示了GmRCA α和GmRCA β的4个反式表达数量性状基因座。这些结果为调控RCA基因提高大豆等植物的光合速率和生长提供了新的途径。
Rubisco activase (RCA) catalyzes the activation of Rubisco in vivo and plays a crucial role in photosynthesis. However, until now, little was known about the molecular genetics of RCA in soybean ( Glycine max), one of the most important legume crops. Here, we cloned and characterized two genes encoding the longer alpha-isoform and the shorter beta-isoform of soybean RCA (GmRCA alpha and GmRCA beta, respectively). The two corresponding cDNAs are divergent in both the translated and 3' untranslated regions. Analysis of genomic DNA sequences suggested that the corresponding mRNAs are transcripts of two different genes and not the products of a single alternatively splicing pre-mRNA. Two additional possible alpha-form RCA-encoding genes, GmRCA03 and GmRCA14, and one additional beta-form RCA-encoding gene, GmRCA11, were also isolated. To examine the function and modulation of RCA genes in soybean, we determined the expression levels of GmRCA alpha and GmRCA beta, Rubisco initial activity, photosynthetic rate, and seed yield in 184 soybean recombinant inbred lines. Correlation of gene expression levels with three other traits indicates that RCA genes could play an important role in regulating soybean photosynthetic capacity and seed yield. Expression quantitative trait loci mapping revealed four trans-expression quantitative trait loci for GmRCA alpha and GmRCA beta. These results could provide a new approach for the modulation of RCA genes to improve photosynthetic rate and plant growth in soybean and other plants.