Glucose deprivation-induced cytotoxicity in drug resistant human breast carcinoma MCF-7/ADR cells: role of c-myc and bcl-2 in apoptotic cell death.
Glucose deprivation-induced cytotoxicity in drug resistant human breast carcinoma MCF-7/ADR cells: role of c-myc and bcl-2 in apoptotic cell death.
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发表时间：
1997-03
影响因子：
4
通讯作者：
Y. Lee;S. Galoforo;C. M. Berns;W. Tong;H. Kim;P. Corry
中科院分区：
文献类型：
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作者：
Y. Lee;S. Galoforo;C. M. Berns;W. Tong;H. Kim;P. Corry
我们研究了葡萄糖剥夺治疗对多重耐药人乳腺癌MCF-7/ADR细胞克隆原性的影响。MCF-7/ADR细胞在无葡萄糖培养基中培养8小时后，其存活率呈指数级下降。这些细胞在葡萄糖剥夺处理8小时后的存活率为1.5 × 10（-3）。显微照片和凝胶电泳数据表明，葡萄糖剥夺诱导的细胞死亡与细胞凋亡有关。western和northern blots的数据显示，在MCF-7/ADR细胞中，无葡萄糖培养基诱导c-myc基因表达。转染c-myc反义寡脱氧核苷酸的MCF-7/ADR细胞对葡萄糖剥夺诱导的细胞凋亡产生抗性。bcl-2基因的过表达保护MCF-7/ADR细胞免于这种凋亡细胞死亡。综上所述，这些结果表明，c-myc表达是无糖培养基诱导细胞凋亡的必要组成部分，而bcl-2可阻止c-myc诱导的凋亡死亡。
We investigated the effect of glucose deprivation treatment on clonogenicity in multidrug-resistant human breast carcinoma MCF-7/ADR cells. Survival of MCF-7/ADR cells decreased exponentially up to 8 hours of incubation in the glucose-free medium. The surviving fraction of these cells for 8 hours of glucose-deprivation treatment was 1.5 x 10(-3). Photomicrographs and gel electrophoresis data suggest that glucose deprivation-induced cell death is associated with apoptosis. Data from western and northern blots showed an induction of c-myc gene expression during treatment with glucose-free medium in MCF-7/ADR cells. MCF-7/ADR cells transfected with c-myc antisense oligodeoxynucleotides became resistant to glucose deprivation-induced apoptosis. Overexpression of bcl-2 gene protected MCF-7/ADR cells from this apoptotic cell death. Taken together, these results indicate that c-myc expression is a necessary component of glucose-free medium induced apoptosis and bcl-2 prevents apoptotic death induced by c-myc.