A 2,2"-bipyridine ligand for incorporation into oligodeoxynucleotides: synthesis, stability and fluorescence properties of ruthenium-DNA complexes.
A 2,2"-bipyridine ligand for incorporation into oligodeoxynucleotides: synthesis, stability and fluorescence properties of ruthenium-DNA complexes.
复制标题
用于掺入寡脱氧核苷酸的 2,2"-联吡啶配体：钌-DNA 复合物的合成、稳定性和荧光特性。
DOI：
10.1093/nar/27.12.2487
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发表时间：
1999
影响因子：
14.9
通讯作者：
McLaughlin,LW
中科院分区：
文献类型：
--
作者：
Wiederholt,K;McLaughlin,LW
A non-nucleoside linker based upon the ligand 2,2′-bipyridine and ethylene glycol is prepared and placed into the backbone of a number of oligonucleo-tides. The bipyridine ligand is reacted withcis-dichloro bis(2,2′-bipyridyl) Ru(ll) to generate the relatively substitutionally inert complex based upon the well-characterized fr/s-2,2′-bipyridyl Ru(ll). The ruthenium-containing DNA complexes exhibited UV and fluorescence characteristics that are consistent with those previously observed for simpletris-2,2′-bipyridyl Ru(ll) complexes. Oligonucleotides containing the ruthenium complex will form both DNA duplexes and triplexes with stabilities that are slightly better than those formed from simple tethered oligonucleotide probes in which the two hybridizing sequences are tethered by simple tri(ethylene glycol) or hexa(ethylene glycol) linkers.
