EXPRESSION OF MONOCYTE CHEMOATTRACTANT PROTEIN-1 (MCP-1) BY RAT ALVEOLAR MACROPHAGES DURING CHRONIC LUNG INJURY
EXPRESSION OF MONOCYTE CHEMOATTRACTANT PROTEIN-1 (MCP-1) BY RAT ALVEOLAR MACROPHAGES DURING CHRONIC LUNG INJURY
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DOI：
10.1165/ajrcmb/9.3.300
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发表时间：
1993-09-01
影响因子：
6.4
通讯作者：
FANTONE, JC
中科院分区：
文献类型：
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作者：
BRIELAND, JK;JONES, ML;FANTONE, JC
利用博莱霉素诱导的大鼠肺纤维化模型，我们确定支气管肺泡灌洗液中单核细胞趋化活性具有时间依赖性。Northern杂交分析显示，诱导肺纤维化后大鼠肺泡巨噬细胞（AMs）单核细胞趋化蛋白-1 (MCP-1) mRNA的表达显著增加。单核细胞趋化活性在损伤大鼠肺提取的AMs条件培养基中也显著增加。这些数据表明，AMs在慢性炎症性肺损伤和肺纤维化发病机制中的一个重要作用是调节肺内单核细胞募集和激活，而单核细胞化学引诱剂包括MCP-1的分泌。
Using a well-characterized model of bleomycin-induced pulmonary fibrosis in the rat, we determined that there was a time-dependent elaboration of monocyte chemotactic activity in bronchoalveolar lavage fluid. Northern hybridization analysis revealed markedly increased expression of rat monocyte chemoattractant protein-1 (MCP-1) mRNA in alveolar macrophages (AMs) from rats following induction of pulmonary fibrosis. Monocyte chemotactic activity was also significantly increased in conditioned media from AMs retrieved from injured rat lungs. These data suggest that one important role of AMs in the pathogenesis of chronic inflammatory lung injury and pulmonary fibrosis is the regulation of monocyte recruitment and activation within the lung secondary to secretion of monocyte chemoattractants including MCP-1.