Complex Reassortment of Multiple Subtypes of Avian Influenza Viruses in Domestic Ducks at the Dongting Lake Region of China
Complex Reassortment of Multiple Subtypes of Avian Influenza Viruses in Domestic Ducks at the Dongting Lake Region of China
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DOI：
10.1128/jvi.00776-13
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发表时间：
2013-09-01
影响因子：
5.4
通讯作者：
Chen, Hualan
中科院分区：
文献类型：
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作者：
Deng, Guohua;Tan, Dan;Chen, Hualan
为了深入了解禽流感病毒（AIV）的生态学，我们从2011年冬季至2012年春季在中国湖南省洞庭湖地区的候鸟迁徙路线上的农场对家鸭进行了主动流感病毒监测。从101个鸭场采集了3，030个鸭拭子样本和1，010个环境样本。我们分离了各种HA亚型的AIV，包括H3、H4、H5、H6、H9、H10、H11和H12。我们对28个代表性分离株的基因组的全部编码序列进行了测序，这些分离株构成了13个特定亚型。当这些菌株之间的系统发育关系进行了检查，我们观察到广泛的重配事件发生。在28株洞庭湖病毒中，共鉴定出21种基因型，涉及6个内部基因。此外，我们还鉴定了从其他国家引进的病毒或病毒基因、来源不明的病毒基因片段和一种新型HA/NA组合。我们的研究结果强调了洞庭湖区养殖家鸭对AIV的发生和进化的重要性，并强调了继续监测该地区家鸭的必要性。
To gain insight into the ecology of avian influenza viruses (AIV), we conducted active influenza virus surveillance in domestic ducks on farms located on the flyway of migratory birds in the Dongting Lake region of Hunan Province, China, from winter 2011 until spring 2012. Specimens comprising 3,030 duck swab samples and 1,010 environmental samples were collected from 101 duck farms. We isolated AIV of various HA subtypes, including H3, H4, H5, H6, H9, H10, H11, and H12. We sequenced the entire coding sequences of the genomes of 28 representative isolates constituting 13 specific subtypes. When the phylogenetic relationships among these isolates were examined, we observed that extensive reassortment events had occurred. Among the 28 Dongting Lake viruses, 21 genotypes involving the six internal genes were identified. Furthermore, we identified viruses or viral genes introduced from other countries, viral gene segments of unknown origin, and a novel HA/NA combination. Our findings emphasize the importance of farmed domestic ducks in the Dongting Lake region to the genesis and evolution of AIV and highlight the need for continued surveillance of domestic ducks in this region.