CHARACTERIZATION OF THE GENOMIC STRUCTURE, CHROMOSOMAL LOCATION AND PROMOTER OF HUMAN PROSTAGLANDIN-H SYNTHASE-2 GENE
CHARACTERIZATION OF THE GENOMIC STRUCTURE, CHROMOSOMAL LOCATION AND PROMOTER OF HUMAN PROSTAGLANDIN-H SYNTHASE-2 GENE
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DOI：
10.1006/bbrc.1994.2167
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发表时间：
1994-08-30
影响因子：
3.1
通讯作者：
WANG, LH
中科院分区：
文献类型：
--
作者：
TAZAWA, R;XU, XM;WANG, LH
前列腺素H合成酶(PGHS)是花生四烯酸转化为前列腺素的限速酶。人类PGHS有两种亚型。PGHS-1是看家基因，而PGHS-2是诱导基因。我们报道了从人噬菌体P1基因组文库中分离出完整的PGHS-2基因及其5‘侧翼区。该基因全长7.5kb，位于第1号染色体上，转录起始点位于ATG起始密码子上游134个碱基。1.8kb的启动子序列含有一个TATA盒和一些潜在的调控元件，包括Cre、NF-kappa B、Sp1和AP2位点。启动子活性研究表明，荧光素酶报告基因5‘侧翼区的前460个核苷酸在佛波酯刺激下能有效地促进人脐静脉内皮细胞荧光素酶报告基因的转录。(C)1994年学术出版社。
Prostaglandin H synthase (PGHS) is the rate-limiting enzyme in the conversion of arachidonic acid to prostanoids. The human PGHS has two isoforms. PGHS-1 is a house keeping gene whereas PGHS-2 is an inducible gene. We reported here the isolation of the entire PGHS-2 gene and its 5'-flanking region from a human bacteriophage P1 genomic library. The gene containing 10 exons is 7.5 kb in length and located at chromosome 1. The transcriptional start site was mapped at 134 bases upstream from the ATG start codon. Nucleotide sequence of 1.8 kb promoter region contains a TATA box and a number of potential regulatory elements including CRE, NF-kappa B, Sp1 and AP2 sites. Studies of the promoter activity showed that the first 460 nucleotides of 5'-flanking region efficiently drove transcription of the luciferase reporter gene in human umbilical vein endothelial cells upon stimulation with phorbor ester. (C) 1994 Academic Press, Inc.