Morphology and distribution of granulomatous inflammation in freshwater ornamental fish infected with mycobacteria
Morphology and distribution of granulomatous inflammation in freshwater ornamental fish infected with mycobacteria
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DOI：
10.1111/j.1365-2761.2010.01202.x
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发表时间：
2010-12-01
影响因子：
2.5
通讯作者：
Pavlik, I.
中科院分区：
文献类型：
--
作者：
Novotny, L.;Halouzka, R.;Pavlik, I.
分支杆菌病是一种由海洋分支杆菌（Mycobacterium marinum，M。gordonae和M.在大多数情况下是偶然的。本研究的目的是描述36种322条淡水观赏鱼的病变形态和分布。通过大体检查和组织病理学检测，188条鱼（58.4%）被诊断为肉芽肿性炎症;经Ziehl-Neelsen染色后，仅在19种鱼中的96条（51.1%）中确定了抗酸杆菌（AFR）。AFR最常见的器官是肾脏（81.2%）、消化道（54.1%）、肝脏（48.2%）、脾脏（45.9%）和皮肤（21.2%），偶尔在鳃（9.4%）和性腺（4.7%）中发现AFR。从4个不同的鱼缸中随机选择14条鱼，通过皮肤、鳃、肌肉组织、消化道、肝、脾和肾的培养检查来研究分枝杆菌感染的分布。在12种鱼类中，M. marinum，M. gordonae、黑脉叶蝉M. fortupe，M. triviale和M.鸟亚种检测到人类分枝杆菌（血清型6和8以及基因型IS 901-和IS 1245+）;在其中5个中记录了由不同分枝杆菌物种引起的混合感染。
Mycobacteriosis in fish is a chronic progressive ubiquitous disease caused by Mycobacterium marinum, M. gordonae and M. fortuitum in most cases. The aim of this study was to describe the morphology and distribution of lesions in 322 freshwater ornamental fish across 36 species. Granulomatous inflammation was diagnosed by gross examination and histopathology testing in 188 fish (58.4%); acid-fast rods (AFR) were determined in only 96 (51.1%) fish from 19 species after Ziehl-Neelsen staining. The most often affected organs with AFR were the kidney (81.2%), digestive tract (54.1%), liver (48.2%), spleen (45.9%) and skin (21.2%); sporadically, AFR were found in the branchiae (9.4%) and gonads (4.7%). In 14 randomly selected fish originating from four different fish tanks, the distribution of mycobacterial infection was studied by culture examination of the skin, gills, muscle tissue, digestive tract, liver, spleen and kidney. In 12 fish, the species M. marinum, M. gordonae, M. fortuitum, M. triviale, and M. avium subsp. hominissuis (serotypes 6 and 8 and genotype IS901- and IS1245+) were detected; mixed infection caused by different mycobacterial species was documented in five of them.