Nanog Dynamics in Mouse Embryonic Stem Cells: Results from Systems Biology Approaches.
Nanog Dynamics in Mouse Embryonic Stem Cells: Results from Systems Biology Approaches.
复制标题
DOI：
10.1155/2017/7160419
复制
发表时间：
2017
影响因子：
4.3
通讯作者：
Marucci L
中科院分区：
文献类型：
--
作者：
Marucci L
小鼠胚胎干细胞（mESCs）来源于囊胚的内部细胞群，是一种具有自我更新能力的多能干细胞，在适当的培养条件下具有向各种细胞类型分化的潜力。越来越多的报告已经发表，揭示的分子机制，协调多能性和细胞命运规范使用联合计算和实验方法。在此，我们回顾了最近的系统生物学方法，以描述mESCs在均匀培养条件下基因表达异质性的原因和功能以及多能性标记物的复杂时间动态。我们特别关注Nanog的动态，它是核心多能性网络和mESC命运的关键调节因子。我们总结了不同实验和建模方法的优势和局限性，并讨论了如何使用各种策略。
Mouse embryonic stem cells (mESCs), derived from the inner cell mass of the blastocyst, are pluripotent stem cells having self-renewal capability and the potential of differentiating into every cell type under the appropriate culture conditions. An increasing number of reports have been published to uncover the molecular mechanisms that orchestrate pluripotency and cell fate specification using combined computational and experimental methodologies. Here, we review recent systems biology approaches to describe the causes and functions of gene expression heterogeneity and complex temporal dynamics of pluripotency markers in mESCs under uniform culture conditions. In particular, we focus on the dynamics of Nanog, a key regulator of the core pluripotency network and of mESC fate. We summarize the strengths and limitations of different experimental and modeling approaches and discuss how various strategies could be used.