Detection of gold nanorods uptake by macrophages using scattering analyses combined with diffusion reflection measurements as a potential tool for in vivo atherosclerosis tracking.
Detection of gold nanorods uptake by macrophages using scattering analyses combined with diffusion reflection measurements as a potential tool for in vivo atherosclerosis tracking.
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DOI：
10.2147/ijn.s86615
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发表时间：
2015
影响因子：
8
通讯作者：
Fixler D
中科院分区：
文献类型：
--
作者：
Ankri R;Melzer S;Tarnok A;Fixler D
在这项研究中，我们报告了一种潜在的无创技术，用于检测易损斑块，使用流式细胞仪(FCM)的散射分析方法结合扩散反射(DR)方法。动脉粥样硬化斑块通常分为两大类：稳定性斑块和易损性斑块。脆弱的斑块富含炎症细胞，主要是巨噬细胞(MΦ)，它们释放酶来分解帽子中的胶原蛋白。该检测方法是基于M-Φ对金纳米棒的吸收。GNR具有独特的光学特性，能够根据其散射特性使用FCM方法进行检测，并基于其独特的吸收特性使用DR方法进行检测。这项工作表明，当M-Φ被标记后，1)流式细胞仪的散射值随任意强度值从1,110增加到4,100而增加了3.7倍；2)DR斜率从平均斜率0.196(仅MΦ)变为平均斜率0.827(MΦ标记GNR)(P<；0.001)。FCM和DR测量的结合为动脉粥样硬化易损斑块的识别提供了一种新的、高灵敏度和非侵入性的新方法，旨在开发一种潜在的活体追踪工具。
In this study, we report a potential noninvasive technique for the detection of vulnerable plaques using scatter analyses with flow cytometry (FCM) method combined with the diffusion reflection (DR) method. The atherosclerotic plaques are commonly divided into two major categories: stable and vulnerable. The vulnerable plaques are rich with inflammatory cells, mostly macrophages (MΦ), which release enzymes that break down collagen in the cap. The detection method is based on uptake of gold nanorods (GNR) by MΦ. The GNR have unique optical properties that enable their detection using the FCM method, based on their scattering properties, and using the DR method, based on their unique absorption properties. This work demonstrates that after GNR labeling of MΦ, 1) the FCM scatter values increased up to 3.7-fold with arbitrary intensity values increasing from 1,110 to 4,100 and 2) the DR slope changed from an average slope of 0.196 (MΦ only) to an average slope of 0.827 (MΦ labeled with GNR) (P<0.001 for both cases). The combination of FCM and DR measurements provides a potential novel, highly sensitive, and noninvasive method for the identification of atherosclerotic vulnerable plaques, aimed to develop a potential tool for in vivo tracking.