Use of time-lapse imaging and dominant negative receptors to dissect the steroid receptor control of neuronal remodeling in Drosophila
Use of time-lapse imaging and dominant negative receptors to dissect the steroid receptor control of neuronal remodeling in Drosophila
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DOI：
10.1242/dev.02191
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发表时间：
2006-01-01
期刊：
影响因子：
4.6
通讯作者：
Truman, JW
中科院分区：
文献类型：
--
作者：
Brown, HLD;Cherbas, L;Truman, JW
在变态过程中，果蝇神经系统的重组部分是通过重塑幼虫神经元进行的。在这项研究中，我们使用体外成像技术和免疫细胞化学追踪胸腹侧神经分泌细胞的重塑。这些神经元的轴突在变态早期修剪它们的幼虫乔木，并且通过分支生长建立更大、更广泛的成年乔木。EcR显性负结构和EcR反向重复结构的表达导致幼虫轴突乔木的修剪缺陷和修剪过程中缺乏丝状伪足，但取决于表达的结构对生长的影响是可变的。表达UAS-EcR-B1（W 650 A）或UAS-EcR-A（W 650 A）的细胞在生长期间缺乏丝状伪足，并在成虫中形成分支不良的幼虫样乔木。表达UAS-EcR-B1（F645 A）、UAS-EcR-B2（W 650 A）或UAS-IR-EcR（核心）的细胞在生长过程中表现出中等的丝状伪足活性和正常的（尽管减少）成体样分支。这些结果是一致的激活与去阻遏通过EcR的连续阶段的神经元重塑的作用，并建议，功能蜕皮激素受体是必要的，但不是所有的，重塑事件。
During metamorphosis, the reorganization of the nervous system of Drosophila melanogaster proceeds in part through remodeling of larval neurons. In this study, we used in-vitro imaging techniques and immunocytochemistry to track the remodeling of the thoracic ventral neurosecretory cells. Axons of these neurons prune their larval arbors early in metamorphosis and a larger, more extensive adult arbor is established via branch outgrowth. Expression of EcR dominant negative constructs and an EcR inverted repeat construct resulted in pruning defects of larval axon arbors and a lack of filopodia during pruning, but showed variable effects on outgrowth depending on the construct expressed. Cells expressing either UAS-EcR-B1(W650A) or UAS-EcR-A(W650A) lacked filopodia during the outgrowth period and formed a poorly branched, larval-like arbor in the adult. Cells expressing UAS-EcR-B1(F645A), UAS-EcR-B2(W650A) or UAS-IR-EcR (core) showed moderate filopodial activity and normal, albeit reduced, adult-like branching during outgrowth. These results are consistent with the role of activation versus derepression via EcR for successive phases of neuronal remodeling and suggest that functional ecdysone receptor is necessary for some, but not all, remodeling events.