Cellular localization of orexin receptors in human pituitary
Cellular localization of orexin receptors in human pituitary
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DOI：
10.1210/jc.86.7.3444
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发表时间：
2001-07-01
影响因子：
5.8
通讯作者：
Beiras, A
中科院分区：
文献类型：
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作者：
Blanco, M;López, M;Beiras, A
食欲素-A和-B是由前食欲素原衍生的下丘脑肽，与刺激食物摄入有关。它们分别作用于名为食欲素受体1（OX 1 R）和食欲素受体2（OX 2 R）的G蛋白受体。在当下。本研究应用RT-PCR和免疫组化技术检测了人垂体中OX 1 R和OX 2 R的表达。RT-PCR结果显示，人垂体中OX 1 R和OX 2 R均出现预期大小的条带。使用组织学技术进行OX 1 R和OX 2 R的细胞定位。通过连续切片，我们证明了OX 1 R存在于嗜酸性、弥散分布的细胞中，这些细胞占腺垂体总细胞群的一半。正如预期的那样，这些细胞显示共表达GH。OX 2 R在垂体前叶的中间部和嗜碱性细胞群中发现，它们共表达ACTH。这些结果被双重免疫荧光技术证实。我们还发现局灶性阳性神经垂体的轴突末梢，更强烈的OX 2 R比OX 1 R。总之，这些结果首次证明，OX 1 R和OX 2 R分别由生长激素细胞和皮质激素细胞表达。
Orexins-A and -B are hypothalamic peptides derived from a precursor called prepro-orexin and relationated with the stimulation of food intake. They act on G protein receptors named orexin receptor 1 (OX1R) and orexin receptor 2 (OX2R), respectively. In the present. study,we used RT-PCR and immunohistochemical techniques to detect the presence of OX1R and OX2R in human pituitary. A band of the expected size for both OX1R and OX2R was shown in human pituitary by RT-PCR. The cellular localization of OX1R and OX2R was carried out using histological techniques. By consecutive sections we demonstrated that OX1R was present in acidophil, diffusely distributed cells, which represent the half of the total adenohypophysis cell population. As was expected, these cells were shown to coexpress GH. OX2R was found in the pars intermedia and in clusters of basophil cells of the anterior pituitary, which coexpress ACTH. These results were confirmed by double immunofluorescence techniques. We also found focal positivity in axon terminals of neurohypophysis, more intense for OX2R than for OX1R. In conclusion, these results demonstrated for the first time that OX1R and OX2R were expressed by somatotrope and corticotrope cells, respectively.