Purinergic Signaling Regulates Cell Proliferation of Olfactory Epithelium Progenitors
Purinergic Signaling Regulates Cell Proliferation of Olfactory Epithelium Progenitors
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DOI：
10.1002/stem.126
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发表时间：
2009-08-01
期刊：
影响因子：
5.2
通讯作者：
Manzini, Ivan
中科院分区：
文献类型：
--
作者：
Hassenkloever, Thomas;Schwartz, Peter;Manzini, Ivan
在嗅上皮（OE）中，持续的神经发生在整个生命过程中得以维持。OE与外部环境直接接触，其细胞不断暴露于病原体和有毒物质。为了保持嗅觉的功能，OE已经进化出永久补充嗅觉受体神经元和支持细胞在自然更替过程中丢失的能力。位于OE最基底部分的细胞群，即所谓的基底细胞（BC），保持这种高度调节的新细胞发生。BC群体被认为包括OE的干细胞和各种祖细胞。近年来，已经确定了一些积极和/或消极地调节OE内的增殖的调节因子，但是对这些调节因子的复杂相互作用和不同上皮细胞类型的作用的透彻理解仍然是虚幻的。结合标记技术、免疫组织化学、电子显微镜、功能性钙成像和溴-2 '-脱氧尿苷掺入测定，我们首次发现嘌呤能受体表达于非洲爪蟾幼体OE的BCs中，核苷酸诱导的Ca 2 +这些细胞中的信号传导参与了OE中细胞周转的调节。我们的数据有助于更好地理解这种调节特别是OE中的细胞更新，以及神经元祖细胞的增殖一般如何调节。干细胞2009;27：2022-2031
In the olfactory epithelium (OE) continuous neurogenesis is maintained throughout life. The OE is in direct contact with the external environment, and its cells are constantly exposed to pathogens and noxious substances. To maintain a functional sense of smell the OE has evolved the ability to permanently replenish olfactory receptor neurons and sustentacular cells lost during natural turnover. A cell population residing in the most basal part of the OE, the so-called basal cells (BCs), keep up this highly regulated genesis of new cells. The population of BCs is thought to include both the stem cells of the OE and various progenitor cells. In recent years a number of regulatory factors that positively and/or negatively regulate the proliferation within the OE have been identified, but a thorough comprehension of the complex interplay of these regulatory factors and the role of the different epithelial cell types is still illusive. Combining labeling techniques, immunohistochemistry, electron microscopy, functional calcium imaging, and a bromo-2'-deoxyuridine incorporation assay, we show for the first time that purinergic receptors are expressed in BCs of the OE of larval Xenopus laevis and that nucleotide-induced Ca2+ signaling in these cells is involved in the regulation of the cell turnover in the OE. Our data contribute to a better understanding of the regulation of the cell turnover in the OE in particular and also of how the proliferation of neuronal progenitor cells is regulated in general. STEM CELLS 2009;27:2022-2031