IMMUNOLOGICAL STUDY OF ANTI-M2 IN ANTIMITOCHONDRIAL ANTIBODY-NEGATIVE PRIMARY BILIARY-CIRRHOSIS
IMMUNOLOGICAL STUDY OF ANTI-M2 IN ANTIMITOCHONDRIAL ANTIBODY-NEGATIVE PRIMARY BILIARY-CIRRHOSIS
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DOI：
10.2169/internalmedicine.34.496
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发表时间：
1995-06-01
影响因子：
1.2
通讯作者：
SATO, N
中科院分区：
文献类型：
--
作者：
KITAMI, N;KOMADA, T;SATO, N
本文研究了抗线粒体抗体（AMA）阴性的原发性胆汁性肝硬化（PBC）患者血清中各种自身抗体的特异性。AMA阴性组平滑肌抗体阳性率显著高于AMA阳性组，而抗核抗体阳性率无显著性差异，酶联免疫吸附试验（ELISA）检测到13%的IgG类抗PDH抗体，而免疫印迹法检测到PBC患者的IgG、伊加和/或IgM类抗M2抗体均为阳性。这些结果表明，IgG和IgM类抗PDH和抗体的四个M2蛋白的检测增加了这种ELISA方法的阳性，和IgG，伊加和IgM类抗M2的四个M2蛋白的免疫印迹检测是有用的AMA阴性PBC患者的诊断。
We investigated the specificity of various autoantibodies in antimitochondrial antibody (AMA)-negative patients with primary biliary cirrhosis (PBC), We examined sera from 144 patients with PBC, 17 of whom were AMA negative by indirect immunofluorescence. The AMA-negative group showed a significantly higher positivity for smooth muscle antibody, but not for antinuclear antibody, as compared with the AMA-positive group, IgG class anti-PDH by enzyme-linked immunosorbent assay (ELISA) mere detected in 13 % of the AMA-negative group, However, all PBC patients showed positive IgG, IgA, and/or IgM class anti-M2 to the four M2 proteins by immunoblotting. These results suggest that the detection of IgG and IgM class anti-PDH and that of antibodies to the four M2 proteins increases the positivity of this ELISA method, and that detection of IgG, IgA, and IgM class anti-M2 to the four M2 proteins by immunoblotting is useful in diagnosing AMA-negative patients with PBC.