Prenatal exposure to benzotriazoles and benzothiazoles and cord blood mitochondrial DNA copy number: A prospective investigation
Prenatal exposure to benzotriazoles and benzothiazoles and cord blood mitochondrial DNA copy number: A prospective investigation
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产前接触苯并三唑和苯并噻唑与脐带血线粒体 DNA 拷贝数：一项前瞻性调查
DOI：
10.1016/j.envint.2020.105920
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发表时间：
2020-10-01
影响因子：
11.8
通讯作者：
Li, Yuanyuan
中科院分区：
文献类型：
--
作者：
Chen, Xiaomei;Zhou, Yanqiu;Li, Yuanyuan
背景：线粒体修复能力有限，对环境毒物敏感。接触苯并三氮唑(BTRs)和苯并噻唑(BTHs)可能会通过氧化应激导致不利的健康后果，这可能会干扰线粒体的功能。然而，暴露于BTS(BTRS和BTHs)对线粒体的影响尚未阐明，尤其是在人类研究中。目的：我们在一个预期出生的队列中，检验了孕期特异性尿BTRs和BTHs浓度与脐血线粒体DNA拷贝数(MtDNAcn)的关系。方法：本研究包括2014-2015年间在武汉参加出生队列的742对母婴，并收集了脐血中BTRs、BTHs和mtDNAcn的尿药浓度数据。用高效液相色谱-串联质谱仪重复测定不同孕期孕妇尿样中BTS的浓度。用实时定量聚合酶链式反应检测脐血中相对线粒体DNAcn。结果：早孕期尿2-甲硫基苯并噻唑(2-MES-BTH)浓度与脐血mtDNAcn呈正相关，差异有统计学意义[百分比变化率(%Delta)=3.97，95%可信区间(CI)：-0.05，8.16，p=0.05]，而妊娠晚期尿2-氨基苯并噻唑浓度与脐血mtDNAcn呈显著负相关(%Delta=-5.89，P<0.05)。95%可信区间：-10.32，-1.24)。妊娠晚期尿1-H-苯并三唑(1-H-BTR)和二甲基三氮唑浓度与脐血mtDNAcn(%Delta=-4.18至-3.23)之间也显示出类似的关联模式。在性别特异性分析中，孕早期母体尿1-H-BTR和孕晚期尿2-MES-BTH与男婴脐血mtDNAcn呈正相关(1-H-BTR交互作用P=0.05，2-MES-BTH交互作用P=0.05)。结论：我们发现了产前接触BTRs和BTHs与脐血mtDNAcn变化相关的证据，并且这种关联被婴儿性别改变了。需要进一步的调查来证实这些发现。
Background: Mitochondria are sensitive to environmental toxicants due to the limited repair capacity. Exposure to benzotriazoles (BTRs) and benzothiazoles (BTHs) may contribute to adverse health outcomes through oxidative stress, which may interfere with mitochondrial function. However, the mitochondrial effects of exposure to BTs (BTRs and BTHs) have not yet been elucidated, particularly in human investigations.Objectives: We examined the associations between trimester-specific urinary BTRs and BTHs concentrations and cord blood mitochondrial DNA copy number (mtDNAcn) in a prospective birth cohort.Methods: The present study included 742 mother-infant pairs who participated in a birth cohort between 2014 and 2015 in Wuhan and had data on urinary concentrations of BTRs and BTHs and mtDNAcn in cord blood. Concentrations of BTs were repeatedly measured in maternal urine samples at different trimesters using high performance liquid chromatography-tandem mass spectrometry. Relative mtDNAcn in umbilical cord blood was analyzed by quantitative real-time polymerase chain reaction. Generalized estimating equations were used to evaluate the associations between BTs exposure across gestation and mtDNAcn in cord blood.Results: In the present study, we observed a positive association between urinary 2-methylthio-benzothiazole (2-MeS-BTH) concentrations in the first trimester and cord blood mtDNAcn, with marginal significance [percent changes (%Delta) = 3.97, 95% confidence interval (CI): -0.05, 8.16, p = 0.05], while urinary 2-amino-benzothiazole concentrations in the third trimester were significantly negatively associated with cord blood mtDNAcn (%Delta = - 5.89, 95% CI: -10.32, -1.24). Similar patterns of associations were demonstrated between urinary 1-H-benzotriazole (1-H-BTR) and xylyltriazole concentrations in the third trimester and cord blood mtDNAcn (%Delta = -4.18 to - 3.23). In sex-specific analysis, we identified that maternal urinary 1-H-BTR in the first trimester and 2-MeS-BTH in the third trimester were positively associated with cord blood mtDNAcn among male infants but not female (P for interaction = 0.05 for 1-H-BTR, P for interaction = 0.05 for 2-MeS-BTH, respectively).Conclusions: We found evidence that prenatal exposure to BTRs and BTHs were associated with cord blood mtDNAcn alternation, and these associations were modified by infant gender. Further investigations are needed to corroborate these findings.