Long-range axonal calcium sweep induces axon retraction
Long-range axonal calcium sweep induces axon retraction
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DOI：
10.1523/jneurosci.0019-08.2008
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发表时间：
2008-04-30
影响因子：
5.3
通讯作者：
Ikegaya, Yuji
中科院分区：
文献类型：
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作者：
Yamada, Ryuji X.;Sasaki, Takuya;Ikegaya, Yuji
轴突引导分子在生长锥中触发一系列局部信号，并引起各种形态反应，包括轴突吸引、排斥、伸长和收缩。然而，除了轴突生长锥外，关于亚细胞区室是否控制轴突的生长知之甚少。我们发现，在离体齿状颗粒细胞中，谷氨酸局部应用于体突区域，而不是轴突本身，诱导轴突快速收缩，在此过程中，钙波从体细胞传播到轴突末端。通过阻断电压敏感钙通道和细胞内钙动力学，钙波和轴突收缩均受到抑制。细胞周围钙离子载体的应用和去极化电流注入的组合诱导轴突钙扫描和轴突收缩。因此，周围环境可以通过远距离细胞内通讯调节轴突行为。
Axon guidance molecules trigger a cascade of local signal in growth cones and evoke various morphologic responses, including axon attraction, repulsion, elongation, and retraction. However, little is known about whether subcellular compartments, other than axonal growth cones, control axon outgrowth. We found that in isolated dentate granule cells, local application of glutamate to the somatodendritic areas, but not the axon itself, induced rapid axon retraction, during which a calcium wave propagated from the somata to the axon terminals. The calcium wave and axon retraction were both inhibited by blockade of voltage-sensitive calcium channels and intracellular calcium dynamics. A combination of perisomatic application of calcium ionophore and depolarizing current injection induced axonal calcium sweep and axon retraction. Thus, perisomatic environments can modulate axon behavior through long-range intracellular communication.