Scanning electron microscopy of virulent and avirulent strains of Xanthomonas campestris pv. oryzae on rice leaves
Scanning electron microscopy of virulent and avirulent strains of Xanthomonas campestris pv. oryzae on rice leaves
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DOI：
10.1094/phyto-74-635
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发表时间：
1984
期刊：
影响因子：
3.2
通讯作者：
T. Mew;I. Mew;J. S. Huang
中科院分区：
文献类型：
--
作者：
T. Mew;I. Mew;J. S. Huang
扫描电子显微镜显示，在所有 cv.-str. 喷雾接种后 1 小时，叶表面细胞数量没有显着差异。组合，但 24 小时后 str 细菌。 PX061，对cvs有剧毒。 TN1 和 CAS 209 在水孔外立即繁殖，一些细菌通过这些孔进入。 PX0101 的细胞，str。它已经失去了毒力，在叶子表面没有明显繁殖，并且似乎嵌入了水孔分泌的一层薄薄的渗出物中，最终密封了这些孔的开口。 PX086菌体对TN1有毒力，但对CAS 209无毒力，在接种后48小时在TN1的水孔上繁殖，但被困在CAS 209的渗出液中。所有 3 个链的细菌细胞。似乎不在这两个品种的气孔上繁殖。结果表明，在不相容的宿主-细菌组合中，细菌被水孔的排泄物固定并抑制分裂。还观察到叶表面细菌繁殖的位点特异性。
Scanning electron microscopy revealed no marked difference in numbers of cells on the leaf surface 1 h after spray-inoculation on all cv.-str. combinations but after 24 h bacteria of str. PX061, which is virulent to cvs. TN1 and CAS 209, multiplied immediately outside the water pores and some bacteria had gained entrance through these pores. Cells of PX0101, a str. that has lost its virulence, did not multiply appreciably on the leaf surface and appeared to be embedded in a thin layer of exudate secreted by the water pores which eventually sealed the opening of these pores. Bacterial cells of PX086, which is virulent to TN1 but avirulent to CAS 209, multiplied on the water pores of TN1 but were trapped in the exudate of CAS 209 48 h after inoculation. Bacterial cells of all 3 strs. seemed not to multiply on stomata of either cultivar. The results suggest that bacteria are immobilized and inhibited from dividing by excretions from water pores in incompatible host-bacteria combinations. Site specificity of bacterial multiplication on the leaf surface was also observed.