Second-generation bioethanol production from phytomass after phytoremediation using recombinant bacteria-yeast co-culture
Second-generation bioethanol production from phytomass after phytoremediation using recombinant bacteria-yeast co-culture
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DOI：
10.1016/j.fuel.2022.124975
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发表时间：
2022
期刊：
影响因子：
7.4
通讯作者：
Tanmoy Roy Tusher;Jui-Jen Chang;Maria Ita Saunivalu;Sosuke Wakasa;Wenhao Li;Chieh-Chen Huang;C. Inoue;Mei-fang Chien
中科院分区：
文献类型：
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作者：
Tanmoy Roy Tusher;Jui-Jen Chang;Maria Ita Saunivalu;Sosuke Wakasa;Wenhao Li;Chieh-Chen Huang;C. Inoue;Mei-fang Chien
收获的超积累植物用于有毒金属的植物修复被认为是相当具有挑战性的可持续管理。超蓄能器的生物质可以成为生物乙醇生产的有吸引力的原料，而去除有毒金属和有效的乙醇转化系统是确保安全和最佳生物乙醇生产所必需的。本研究旨在开发一种兼容且高效的综合生物处理系统（CBP），用于从砷(As)-超蓄积体pteris vittata的植物质中生产生物乙醇。利用含有6个纤维素酶基因的重组克菲尔酵母（KR7菌株）和2个不同排列方式携带纤维素酶基因的重组枯草芽孢杆菌（1型和2型菌株）进行糖化发酵。采用一步法（仅KR7菌株）和共培养法（枯草芽孢杆菌+ KR7菌株）对葡萄球菌进行研究。还原糖实验结果表明，KR7菌株的糖化能力高于这两种菌株。subtilisstrains。而b的共同文化。subtilist2型菌株与KR7菌株的乙醇产率最高，达到48.5%。三维培养后的葡萄团。此外，采用摇匀法进行简单预处理。添加1% hno3的vittaa可有效去除植物质中80%以上的As，表明该方法可用于高效提取As，确保高效无毒生产乙醇。我们的研究结果证实，所开发的重组细菌-酵母共培养具有通过CBP从as -超积累物衍生的植物质中获得最佳生物乙醇的潜力。
Sustainable management of harvested hyperaccumulating plants used in phytoremediation of toxic metals is considered quite challenging. The biomass of hyperaccumulators can be an attractive feedstock for bioethanol production, while removal of toxic metals and efficient ethanol conversion systems are necessary to ensure safe and optimum bioethanol production. This study aimed to develop a compatible and efficient consolidated bioprocessing system (CBP) to produce bioethanol from phytomass of arsenic (As)-hyperaccumulatorPteris vittata. A recombinant kefir yeastKluyveromyces marxianus(KR7 strain) contained six cellulase genes and two recombinantBacillus subtilis(Type 1 and Type 2 strains) carrying cellulosomal genes in different arrangements were used, and the saccharification and fermentation ofP. vittataphytomass were investigated through one-step (KR7 strain only) and co-culture (B. subtilis+ KR7 strain) methods. The results of reducing sugar showed that KR7 strain possesses higher saccharification ability than bothB. subtilisstrains. While the co-culture ofB. subtilisType 2 strain with KR7 strain gave the highest ethanol yield, which achieved 48.5% ethanol yield fromP. vittataphytomass after 3 d cultivation. In addition, simple pretreatment by shakingP. vittatawith 1% of HNO3efficiently removed more than 80% of As from the phytomass, suggesting its applicability and feasibility for efficient As extraction to ensure efficient and non-toxic ethanol production. Our results confirm that the developed recombinant bacteria-yeast co-culture possesses a great potential for optimum bioethanol production from As-hyperaccumulators-derived phytomass via CBP.