Microwave-assisted synthesis of highly water-soluble graphene towards electrical DNA sensor.
Microwave-assisted synthesis of highly water-soluble graphene towards electrical DNA sensor.
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DOI：
10.1039/c0nr00562b
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发表时间：
2010-12
期刊：
影响因子：
6.7
通讯作者：
B. Choi;Hoseok Park;Min Yang;Y. Jung;S. Y. Lee;W. Hong;T. Park
中科院分区：
文献类型：
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作者：
B. Choi;Hoseok Park;Min Yang;Y. Jung;S. Y. Lee;W. Hong;T. Park
石墨烯片在电化学器件中具有实际应用的潜力，但石墨烯片聚集的关键问题阻碍了其发展。在这里，我们展示了一种通过微波辅助磺化 (MAS) 使用水溶性磺化还原氧化石墨烯 (SRGO) 片制造 DNA 传感器装置的简便且自下而上的方法，显示出增强的灵敏度、可靠性和低检测限。实现这些性能的关键是SRGO的制造，其中MAS方法使SRGO能够高度分散在水中（10 mg mL(-1)），因为功能化反应3分钟内产生酸性磺化基团。通过平坦单层石墨烯片和 DNA 之间的静电相互作用制备的水溶性 SRGO-DNA (SRGOD) 杂化物由于 SRGOD 独特的电特性，适用于制造电 DNA 传感器设备。通过使用互补 DNA、单碱基错配 DNA 和非互补 DNA 检测 DNA 杂交，证明了 SRGOD 传感器的高传感性能，结果显示比基于还原氧化石墨烯的 DNA 传感器具有更高的灵敏度和更低的检测限。使用 SRGOD 简单轻松地制造 DNA 传感器设备有望为使用微型传感器进行 DNA 杂交电检测提供有效的方法，而无需对传感器进行劳动密集型标记和复杂的测量设备。
Graphene sheets have the potential for practical applications in electrochemical devices, but their development has been impeded by critical problems with aggregation of graphene sheets. Here, we demonstrated a facile and bottom-up approach for fabrication of DNA sensor device using water-soluble sulfonated reduced graphene oxide (SRGO) sheets via microwave-assisted sulfonation (MAS), showing enhanced sensitivity, reliability, and low detection limit. Key to achieving these performances is the fabrication of the SRGOs, where the MAS method enabled SRGOs to be highly dispersed in water (10 mg mL(-1)) due to the acidic sulfonated groups generated within 3 min of the functionalization reaction. The water-soluble SRGO-DNA (SRGOD) hybrids prepared by electrostatic interactions between a flat single layer of graphene sheets and DNAs are suitable for fabrication of electrical DNA sensor devices because of the unique electrical characteristics of SRGODs. The high sensing performance of SRGOD sensors was demonstrated with detection of DNA hybridization using complementary DNAs, single base mismatched DNAs, and noncomplementary DNAs, with results showing higher sensitivity and lower detection limit than those of reduced graphene oxide-based DNA sensors. Simple and easy fabrication of DNA sensor devices using SRGODs is expected to provide an effective way for electrical detection of DNA hybridization using miniature sensors without the labor-intensive labeling of the sensor and complex measurement equipment.