Improvement of sugar analysis sensitivity using anion-exchange chromatography-electrospray ionization mass spectrometry with sheath liquid interface.
Improvement of sugar analysis sensitivity using anion-exchange chromatography-electrospray ionization mass spectrometry with sheath liquid interface.
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DOI：
10.1016/j.chroma.2014.09.019
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发表时间：
2014-10
期刊：
影响因子：
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通讯作者：
Xian-Bing Xu;Ding-Bo Liu;Xiaobing Guo;Shujuan Yu;P. Yu
中科院分区：
文献类型：
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作者：
Xian-Bing Xu;Ding-Bo Liu;Xiaobing Guo;Shujuan Yu;P. Yu
报道了一种新型的电喷雾离子化（ESI）质谱与高效阴离子交换色谱（HPAEC）联用接口。鞘液由含0.05%乙酸的异丙醇中的50 mM NH 4Ac组成，以3 μL/min的流速注入电喷雾探针尖端，需要更少的ESI源清洁，并促进单碳水化合物和二碳水化合物的有效电离。结果表明，使用鞘液界面而不是T型接头允许挥发性铵盐代替非挥发性金属盐作为改进糖ESI信号的改性剂。在ESI源中的单-和二-碳水化合物的有效电离受鞘液性质如缓冲液浓度和有机溶剂类型的影响。将HPAEC-ESI-MS用于果胶中单糖的分析，特别是共洗脱糖的分析，并对其性能进行了评价。通过鞘液界面添加补充溶液被证明是用于增强使用HPAEC-ESI-MS分析的糖的强度的有效工具。
A novel interface that enables high-performance anion-exchange chromatography (HPAEC) to be coupled with electrospray ionization (ESI) mass spectrometry (MS) is reported. A sheath liquid consisting of 50 mM NH4Ac in isopropanol with 0.05% acetic acid, infused at a flow rate of 3 μL/min at the tip of the electrospray probe, requires less ESI source cleaning and promotes efficient ionization of mono- and di-carbohydrates. The results suggest that use of a sheath liquid interface rather than a T-joint allows volatile ammonium salts to replace non-volatile metal salts as modifiers for improving sugar ESI signals. The efficient ionization of mono- and di-carbohydrates in the ESI source is affected by the sheath liquid properties such as buffer concentration and type of organic solvent. HPAEC-ESI-MS was used for the analysis of monocarbohydrates in pectins, particularly co-eluted sugars, and the performance was evaluated. Addition of a make-up solution through the sheath liquid interface proved to be an efficient tool for enhancing the intensities of sugars analyzed using HPAEC-ESI-MS.