Effects of different intensities of endurance exercise on oxidative stress and antioxidant capacity
Effects of different intensities of endurance exercise on oxidative stress and antioxidant capacity
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DOI：
10.7600/jpfsm.1.183
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发表时间：
2012-05
期刊：
影响因子：
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通讯作者：
Masaki Takahashi;Katsuhiko Suzuki;H. Matoba;S. Sakamoto;S. Obara
中科院分区：
文献类型：
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作者：
Masaki Takahashi;Katsuhiko Suzuki;H. Matoba;S. Sakamoto;S. Obara
本研究的目的是探讨三种不同强度的运动对氧化应激和抗氧化能力的影响。八名健康男性受试者在自行车测力计上进行了三种不同强度的运动，持续 20 分钟：(1) 70% 无氧阈值 (AT)（光强度，LI）、(2) 100% AT（中等强度，MI）和 (3) 130% AT（高强度，HI）。对照（C）试验是在静息条件下进行的。运动前、运动后立即和运动后 30 分钟采集血样。仅在 HI 试验中，运动后立即（P<0.01）和 30 分钟（P<0.05）血浆活性氧代谢物衍生物 (d-ROM) 浓度显着高于运动前。此外，在 HI 试验中，运动后血浆 trolox 当量抗氧化能力显着升高（P<0.01）。而且，在 MI（P<0.05）和 HI 试验（P<0.01）中，运动后血浆谷胱甘肽过氧化物酶 (GPX) 活性显着高于运动前。这些数据表明，高于 AT 水平的 20 分钟踩踏运动可能会增加 ROS 的产生，而内源酶活性（尤其是 GPX 活性）或非酶抗氧化剂可能会调节运动诱导的 ROS 产生。因此，AT水平下的运动对年轻人造成的氧化应激损伤非常小。
The purpose of this study was to investigate the effects of three different intensities of exercise on oxidative stress and antioxidant capacity. Eight healthy male subjects performed three different intensities of exercise for 20 minutes: (1) 70% anaerobic threshold (AT) (light intensity, LI), (2) 100% AT (moderate intensity, MI), and (3) 130% AT (high intensity, HI) on a cycle ergometer. A control (C) trial was conducted under resting conditions. Blood samples were taken pre-exercise, immediately and 30 min after exercise. In the HI trial only, the concentrations of plasma derivatives of reactive oxygen metabolites (d-ROMs) were significantly higher immediately (P<0.01) and 30 min after exercise (P<0.05) than at preexercise. Furthermore, plasma trolox equivalent antioxidant capacity was significantly higher immediately after exercise in the HI trial (P<0.01). And, plasma glutathione peroxidase (GPX) activity was significantly higher immediately after exercise than at pre-exercise in the MI (P<0.05) and HI trials (P<0.01). These data suggest that 20 min of pedaling exercise above the AT level may increase ROS production, while endogenous enzyme activity (especially GPX activity) or non-enzymatic antioxidants may modulate exercise-induced ROS generation. Thus, exercise under the AT level induces very little oxidative stress damage in young people.