Nicotine self-administration with menthol and audiovisual cue facilitates differential packaging of CYP2A6 and cytokines/chemokines in rat plasma extracellular vesicles.
Nicotine self-administration with menthol and audiovisual cue facilitates differential packaging of CYP2A6 and cytokines/chemokines in rat plasma extracellular vesicles.
复制标题
DOI：
10.1038/s41598-021-96807-5
复制
发表时间：
2021-08-30
影响因子：
4.6
通讯作者：
Kumar S
中科院分区：
文献类型：
--
作者：
Kumar A;Sinha N;Haque S;Kodidela S;Wang T;Martinez AG;Chen H;Kumar S
在这项研究中，我们研究了静脉注射尼古丁与薄荷醇和视听线索是否调节大鼠血浆细胞外小泡(EVS)中尼古丁代谢的CYP2A6、氧化应激调节剂和细胞因子/趋化因子。我们将大鼠分为三组：(A)视听提示(AV)、(B)薄荷醇和(C)薄荷醇和视听提示(AV)。我们发现，在自我给予尼古丁和薄荷脑和AV提示后，血浆EVS中CD9的水平增加。此外，在薄荷醇和AV提示下，自我给予尼古丁后，血浆EVS中CYP2A6的表达显著增加。然而，尽管SOD1和过氧化氢酶有上升的趋势，但没有发现有统计学意义的增加，而血浆和血浆EV的总抗氧化能力被发现显著增加，在自身给予尼古丁和薄荷醇和AV线索后获得。在细胞因子和趋化因子分析中，我们发现，在EVS中自我给予尼古丁与薄荷脑和AV线索并完全包装IL-1β后，单核细胞趋化蛋白-1的水平显著增加。综上所述，这项研究提供了证据，表明尼古丁对薄荷醇和AV信号的反应可以包装血浆EV中细胞因子/趋化因子水平的变化，这些变化可能有助于细胞间通信、尼古丁代谢和吸烟引起的炎症。
In this study, we investigated whether intravenously self-administered nicotine with menthol and audiovisual cue modulates nicotine-metabolizing CYP2A6, oxidative stress modulators, and cytokines/chemokines in plasma extracellular vesicles (EVs) in rats. We assigned rats to self-administered nicotine with: (a) audiovisual cue (AV), (b) menthol, and (c) menthol and AV cue. We found increased levels of CD9 in plasma EVs after self-administered nicotine with menthol and AV cue. Moreover, expression of CYP2A6 in plasma EVs was significantly increased after self-administered nicotine in response to menthol and AV cue. However, despite an upward trend on SOD1 and catalase, increase was not found to be statistically significant, while total antioxidant capacity was found to be significantly increased in plasma and plasma EVs obtained after self-administered nicotine with menthol and AV cue. Among cytokine and chemokine profiling, we found a significant increase in the levels of MCP-1 after self-administered nicotine with menthol and AV cue and complete packaging of IL-1β in EVs. Taken together, the study provides evidence that nicotine in response to menthol and AV cues can package altered levels of CYP2A6, and cytokines/chemokines in plasma EVs that may contribute to cell–cell communication, nicotine metabolism, and inflammation upon cigarette smoking.
登录
查看更多内容
影响因子：
3.7
作者：
Jin M;Kumar A;Kumar S
通讯作者：
Kumar S
影响因子：
2.2
作者：
Ande A;Sinha N;Rao PS;McArthur CP;Ayuk L;Achu PN;Njinda A;Kumar A;Kumar S
通讯作者：
Kumar S
影响因子：
12.7
作者：
Wang, Richard J.;Bhadriraju, Sudhamayi;Glantz, Stanton A.
通讯作者：
Glantz, Stanton A.
DOI：
10.1038/npp.2011.299
发表时间：
2012-04
期刊：
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology
影响因子：
--
作者：
通讯作者：
--
影响因子：
16
作者：
Fujita Y;Araya J;Ito S;Kobayashi K;Kosaka N;Yoshioka Y;Kadota T;Hara H;Kuwano K;Ochiya T
通讯作者：
Ochiya T