CYTOKINES AND THE ACUTE-PHASE RESPONSE TO INFLUENZA-VIRUS IN MICE
CYTOKINES AND THE ACUTE-PHASE RESPONSE TO INFLUENZA-VIRUS IN MICE
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DOI：
10.1152/ajpregu.1995.268.1.r78
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发表时间：
1995-01-01
影响因子：
2.8
通讯作者：
KLUGER, MJ
中科院分区：
文献类型：
--
作者：
CONN, CA;MCCLELLAN, JL;KLUGER, MJ
本研究表征了小鼠鼻内接种两种致死率不同的小鼠适应性流感病毒后急性期反应的选定方面。给予140个噬斑形成单位（PFU）的病毒（58%存活）的小鼠在6天内逐渐减少食物和水的摄入量至接近零;幸存者然后缓慢增加摄入量。这些行为的下降与体温和一般运动活动的降低平行，并与肺灌洗液中白细胞介素-6（IL-6）、肿瘤坏死因子-α和干扰素的活性升高相关。这些细胞因子的循环水平没有增加。在55，000 PFU病毒（100%死亡率）后，食物和水的摄入量在48 h内降至接近零，体温和运动活性显著降低，肺灌洗液中IL-1和IL-6的活性升高。这些数据表明，肺中的细胞因子活性在支持细胞因子可以介导小鼠在急性流感感染期间的行为和生理变化的假设的时间范围内升高。
This study characterized selected aspects of the acute phase response after intranasal inoculation of mice with two doses of mouse-adapted influenza virus differing in lethality. Mice given 140 plaque-forming units (PFU) of virus (58% survival) gradually decreased food and water intake to nearly zero over 6 days; survivors then slowly increased intakes. Declines in these behaviors were parallel to decreases in body temperature and general locomotor activity and were associated with elevated activities of interleukin-6 (IL-6), tumor necrosis factor-alpha, and interferons in lung lavage fluid. Circulating levels of these cytokines were not increased. After 55,000 PFU of virus (100% mortality), food and water intake fell to near zero within 48 h, temperature and locomotor activity decreased significantly, and activities of IL-1 and IL-6 were elevated in lung lavage fluid. These data show that cytokine activities in the lungs are elevated in a time frame that supports the hypothesis that cytokines could mediate behavioral and physiological changes in mice during acute influenza infections.