In vitro selection of proteins with desired characteristics using mRNA-display.
In vitro selection of proteins with desired characteristics using mRNA-display.
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DOI：
10.1016/j.ymeth.2012.11.004
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发表时间：
2013-03-15
期刊：
影响因子：
4.8
通讯作者：
Liu, Rihe
中科院分区：
文献类型：
--
作者：
Valencia, C. Alexander;Zou, Jianwei;Liu, Rihe
mRNA-display is an amplification-based, iterative rounds of in vitro protein selection technique that circumvents a number of difficulties associated with yeast two-hybrid and phage display. Because of the covalent linkage between the genotype and the phenotype, mRNA-display provides a powerful means for reading and amplifying a peptide or protein sequence after it has been selected from a library with very high diversity. The purpose of this article is to provide a summary of the field and practical framework of mRNA-display-based selections. We summarize the advantages and limitations of selections using mRNA-display as well as the recent applications, namely, the identification of novel affinity reagents, target-binding partners, and enzyme substrates from synthetic peptide or natural proteome libraries. Practically, we provide a detailed procedure for performing mRNA-display-based selections with the aim of identifying protease substrates and binding partners of a target protein. Furthermore, we describe how to confirm the function of the selected protein sequences by biochemical assays and bioinformatic tools.
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DOI：
10.1073/pnas.0509219103
发表时间：
2006-03-21
影响因子：
11.1
作者：
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通讯作者：
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影响因子：
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DOI：
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发表时间：
1993-04-15
影响因子：
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作者：
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通讯作者：
SEIDEMANN, E
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作者：
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通讯作者：
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