CELL-CULTURE ON A THERMORESPONSIVE POLYMER SURFACE
CELL-CULTURE ON A THERMORESPONSIVE POLYMER SURFACE
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DOI：
10.1038/nbt0990-854
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发表时间：
1990-09-01
期刊：
影响因子：
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通讯作者：
YOSHIZATO, K
中科院分区：
文献类型：
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作者：
TAKEZAWA, T;MORI, Y;YOSHIZATO, K
我们已经使用了一种热响应聚合物，聚-N-异丙基丙烯酰胺（PNI-PAAm），作为一种基质的人真皮成纤维细胞的培养，通过共轭它与胶原蛋白。细胞在基质上贴壁、铺展、生长良好，表明聚合物对细胞无毒性。PNIPAAm在较低临界溶解温度（LCST;约32 ℃）以上不溶于水。C）并在LCST以下可逆溶解。利用这种转化，在含有PNIPAAm超过LCST的基质上培养的单层成纤维细胞通过简单地将温度降低到低于LCST而完全从基质上分离，而不使用常规的分离剂如胰蛋白酶和EDTA。脱落的细胞片逐渐聚集，最终形成多细胞球体。这种聚合物可以为细胞培养提供方便和潜在有用的技术。
We have used a thermo-responsive polymer, poly-N-isopropyl acrylamide (PNI-PAAm), as a substratum for the culture of human dermal fibroblasts by conjugating it with collagen. The cells attached well, spread, and grew on the substratum, indicating that the polymer has no toxicity towards the cells. PNIPAAm is insoluble in water over the lower critical solution temperature (LCST; about 32.degree. C) and reversibly solubilized below the LCST. Taking advantage of this conversion, monolayered fibroblasts cultured on the substratum containing the PNIPAAm over the LCST, were completely detachable from the substratum by simply lowering the temperature below the LCST, without the use of conventional detaching agents such as trypsin and EDTA. The detached cell sheet gradually aggregated and finally formed a multicellular spheroid. This polymer may provide a convenient and potentially useful technology for cell culture.