EDAC fixation increases the demonstrability of biogenic amines in the unicellular Tetrahymena:: A flow cytometric and confocal microscopic comparative analysis
EDAC fixation increases the demonstrability of biogenic amines in the unicellular Tetrahymena:: A flow cytometric and confocal microscopic comparative analysis
复制标题
DOI：
10.1016/j.cellbi.2005.12.011
复制
发表时间：
2006-04-01
影响因子：
3.9
通讯作者：
Pállinger, É
中科院分区：
文献类型：
--
作者：
Csaba, G;Kovács, P;Pállinger, É
早期的实验证明，在四膜虫中存在排名较高的动物的激素。在本实验中，两种固定剂是常用的多聚甲醛和Panula等人推荐的碳二亚胺EDAC。为了组胺的免疫细胞化学，比较了四膜虫的组胺和5-羟色胺的免疫细胞化学，并用流式细胞仪和共聚焦显微镜进行了比较。EDAC固定后，两种激素水平均显著升高；5-羟色胺几乎翻了一番，组胺增加了五倍多。共聚焦显微镜图像更清晰，激素定位更容易。这些结果支持了早期关于四膜虫中存在这些激素的观察结果，并指出了EDAC固定在免疫细胞化学中展示这些激素的优势。(C)2006年国际细胞生物学联合会。爱思唯尔有限公司出版。保留所有权利。
Earlier experiments demonstrated the presence of hormones of the higher ranked animals in Tetrahymena. In the present experiments two fixatives, paraformaldehyde, which is commonly used and a carbodiimide, EDAC that was recommended by Panula et al. for the immunocytochemistry of histamine, are compared in Tetrahymena for the demonstration of histamine and serotonin by using flow cytometry and confocal microscopy. Both hormone levels were significantly higher after EDAC fixation; serotonin almost doubled and histamine was more than fivefold. The confocal microscopic pictures were clearer and the hormones' localization was easier. The results support earlier observations on the presence of these hormones in Tetrahymena and points to the advantage of EDAC fixation for demonstrating these hormones immunocytochemically. (c) 2006 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.