FURTHER EVIDENCE FOR PAIN-RELATED BEHAVIORS IN A MODEL OF UNILATERAL PERIPHERAL MONONEUROPATHY
FURTHER EVIDENCE FOR PAIN-RELATED BEHAVIORS IN A MODEL OF UNILATERAL PERIPHERAL MONONEUROPATHY
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DOI：
10.1016/0304-3959(90)90022-6
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发表时间：
1990-05-01
期刊：
影响因子：
7.4
通讯作者：
GUILBAUD, G
中科院分区：
文献类型：
--
作者：
ATTAL, N;JAZAT, F;GUILBAUD, G
最近在大鼠中描述了产生疼痛相关疾病的实验性周围神经病模型。本研究的目的是调查，使用不同的和可量化的行为的方法，在动物的异常疼痛相关的感觉。神经病变是由4根结扎线松散地绑在坐骨总神经周围产生的。6-8手术后10天，大多数大鼠在坐骨神经结扎同侧表现出疼痛相关的病症，其在手术后2周达到最大。机械伤害性刺激（捏后爪）显示所有动物均出现痛觉过敏。当用对爪加压的发声阈值测试进行测试时，大鼠也表现出异常性疼痛（平均发声阈值为65.5 ± 0.5）。3.6%，P < 0.01，n = 95）。使用热（40、42、44、46 ° C）的测试。C）和冷（10. C）刺激（将大鼠的后爪浸入浴中，直到观察到其挣扎）表明对有害热的痛觉过敏（在46 ℃的温度下浸入持续时间（ID）减少30%）。C），以及对非伤害性热（斗争阈值温度降低30%）和冷刺激（ID降低40%）的异常性疼痛。此外，动物在自然环境中受影响的后爪的自发姿势显示出改变，这表明“自发"的疼痛相关行为（从用于福尔马林试验的技术得到的并编号为0-5的平均”疼痛"等级为2.8 ± 1.5。0.4，P < 0.01，n = 12）。最后，在施加于受损但非受损爪的非伤害范围内的热刺激后，观察到对机械刺激的敏化反应。疼痛相关疾病的时间进程是可比的，无论是行为测试，术后2个月恢复。这些结果清楚地表明，神经病变在大鼠中产生异常的疼痛相关疾病，这让人想起在一些人类神经病变中观察到的那些。
A model of experimental peripheral neuropathy producing pain-related disorders has recently been described in the rat. The present study aimed to investigate, using a different and quantifiable behavioural approach, the abnormal pain-related sensations in the animals. The neuropathy was produced by 4 ligatures tied loosely around the common sciatic nerve. 6-8 days after surgery, most of the rats exhibited pain-related disorders ipsilateral to the sciatic ligation, which became maximal 2 weeks after surgery. Mechanical noxious stimulation (pinching of the hind paw) revealed hyperalgesia in all the animals. Rats also exhibited allodynia when tested with the vocalization threshold test to paw pressor (mean vocalization thresholds were 65.5 .+-. 3.6% of the preoperative control, P < 0.01, n = 95). Tests using heat (40, 42, 44, 46.degree. C) and cold (10.degree. C) stimulation (immersion of the rat''s hind paw in a bath until it was observed to struggle) indicated hyperalgesia to noxious heat (decrease of 30% in the immersion duration (ID) at a temperature of 46.degree. C), and allodynia to non-noxious heat (decrease of 30% in the temperature of the struggle threshold) and to cold stimulation (decrease by 40% in the ID). In addition, the animals showed modifications in the spontaneous postures of the affected hind paw in a natural setting, suggesting a ''spontaneous'' pain-related behaviour (the mean ''pain'' rating, derived from the technique used for the formalin test and numbered 0-5, was 2.8 .+-. 0.4, P < 0.01, n = 12). Lastly, sensitized responses were observed to mechanical stimulation after thermal stimulation in the non-noxious range applied to the lesioned but not the non-lesioned paw. The time course of pain-related disorders was comparable whatever the behavioural test, with recovery 2 months after surgery. These results clearly show that the neuropathy produces abnormal pain-related disorders in the rat, which are reminiscent of those observed in some human neuropathies.