Molecular characterization of a wheat -: Thinopyrum ponticum partial amphiploid and its derivatives for resistance to leaf rust
Molecular characterization of a wheat -: Thinopyrum ponticum partial amphiploid and its derivatives for resistance to leaf rust
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作者：
Li, HJ;Chen, O;Chu, CC
叶锈病（由小麦锈病引起）每年在世界上大多数小麦种植区发生。石竹（石竹）z。刘荣成。Wang提供了几种抗叶锈病基因来保护小麦免受这种真菌疾病的侵害。以小麦（Triticum aestivum L. ern Thell.）与小麦（Triticum aestivum L. n Thell.）杂交，获得了具有冬季生长习性的3个染色体替代系Ji806、Ji807、Ji859和2个染色体附加系Ji791、Ji924。Ponticum部分两倍体系693。这些品系对来自中国的叶锈病分离株具有抗性。利用Lr24基因连锁的J09-STS标记进行序列标记位点（STS）分析，发现部分两倍体系693和所有替代系和附加系均携带Lr24基因。在中国春小麦T aestivum （ABD基因组，2n = 42）基因组DNA存在的情况下，以糙皮假芽孢菌（m.b eib） a . Love （St基因组，2n = 14）的总基因组DNA为探针，对染色体制备进行了基因组原位杂交（GISH）分析。GISH分析表明，这些系有一对染色体显示典型的J(s)基因组染色体模式。这表明携带Lr24基因的染色体属于Th的Js基因组。ponticum。在部分两倍体系693中，除40条小麦染色体外，还分化出8条J(s)和8条J染色体。由于Lr24大部分来源的籽粒颜色为红色，所有这些替代系和附加系的籽粒颜色都是白色的，加上其中一些系的蛋白质含量很高，使它们成为冬小麦育种计划中非常有用的供体来源。
Leaf rust (caused by Puccinia triticina Eriks.) occurs annually in most wheat-growing areas of the world. Thinopyrum ponticum (Podp.) Z.-W. Liu & R.-C. Wang has provided several leaf rust resistance genes to protect wheat from this fungal disease. Three chromosome substitution lines, Ji806, Ji807, and Ji859, and two chromosome addition lines, Ji791 and Ji924, with a winter growing habit were developed from crosses between wheat (Triticum aestivum L. ern Thell.) and the wheat - Th. ponticum partial amphiploid line 693. These lines were resistant to leaf rust isolates from China. Sequence-tagged site (STS) analysis with the J09-STS marker, which is linked to the gene Lr24, revealed that the partial amphiploid line 693 and all of the substitution and addition lines carried gene Lr24. Genomic in situ hybridization (GISH) analysis was carried out on chromosome preparations using total genomic DNA from Pseudoroegneria strigosa (M. Bieb) A. Love (St genome, 2n = 14) as a probe in the presence of total genomic DNA from T aestivum 'Chinese Spring' wheat (ABD genomes, 2n = 42). The GISH analysis demonstrated that these lines had a pair of chromosomes displaying the typical pattern of a J(s) genome chromosome. This indicates that the chromosome that carries gene Lr24 belonged to the Js genome of Th. ponticum. In addition to 40 wheat chromosomes, eight J(s) and eight J genome chromosomes were also differentiated by GISH in the partial amphiploid line 693. Since most sources of Lr24 have a red grain color, the white-colored seeds in all of these substitution and addition lines, together with high protein content in some of the lines, make them very useful as a donor source for winter wheat breeding programs.