ANALYSIS OF STRUCTURAL-CHANGES IN THE STRIA VASCULARIS FOLLOWING CHRONIC GENTAMICIN TREATMENT
ANALYSIS OF STRUCTURAL-CHANGES IN THE STRIA VASCULARIS FOLLOWING CHRONIC GENTAMICIN TREATMENT
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DOI：
10.1016/0378-5955(87)90195-x
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发表时间：
1987-01-01
期刊：
影响因子：
2.8
通讯作者：
DAVIES, SJ
中科院分区：
文献类型：
--
作者：
FORGE, A;WRIGHT, A;DAVIES, SJ
用定量方法研究了慢性庆大霉素治疗后血管纹的变化。给予白化病豚鼠100 mg庆大霉素。kg-1.第-1天皮下注射10天。对最后一次注射后1小时或4周处死的给药动物的肺组织与注射生理盐水的对照动物的肺组织进行比较。从扫描电子显微镜照片中确定了条纹宽度（Reissner膜的螺旋突起）和跨条纹的边缘细胞（MC）数量。从薄切片中获得了Strial厚度（内淋巴表面到螺旋韧带）和血管成分（MC，中间细胞（IC），基底细胞（BC）和毛细血管）的体积分数。最后一次注射后1小时，MC和IC的定性变化明显。在治疗后4周，边缘细胞的数量有一个小的，但具有统计学意义的减少，而视网膜厚度有一个非常显著的减少。这几乎完全是由于MC的体积分数（即收缩）的高度显著降低。IC的体积分数增加，但这可以解释为MC收缩;在允许减少的厚度，IC占据的体积不变。因此，在慢性庆大霉素治疗后，纹受到影响，但显著的进行性和永久性结构效应仅限于边缘细胞。
Changes in the stria vascularis following chronic gentamicin treatment have been examined using quantitative methods. Albino guinea pigs were given gentamicin at 100 mg .cntdot. kg-1 .cntdot. day-1 subcutaneously for 10 days. Comparisons were made of strial tissue from the treated animals sacrificed either 1 h or 4 weeks following the last injection with that from saline-injected controls. Strial width (spiral prominence to Reissner''s membrane) and marginal cell (MC) number across the stria were determined from scanning electron micrographs. Strial thickness (endolymphatic surface to spiral ligament) and the volume fractions of the strial components (MCs, intermediate cells (ICs), basal cells (BCs) and capillaries) were derived from thin sections. Qualitative changes to both MCs and ICs were apparent 1 h after the last injection. At four weeks post-treatment, there was a small, but statistically significant, decrease in the number of marginal cells and a highly significant decrease in strial thickness. This was almost entirely due to a highly significant decrease in the volume fraction of MCs (i.e. shrinkage). The volume fraction of ICs was increased but this could be accounted for by MC shrinkage; after allowing for the reduction in strial thickness, the volume occupied by ICs was unchanged. Thus, following chronic gentamicin treatment, the stria is affected but significant progressive and permanent structural effects are confined to the marginal cells.