Using the CPTAC Assay Portal to Identify and Implement Highly Characterized Targeted Proteomics Assays.
Using the CPTAC Assay Portal to Identify and Implement Highly Characterized Targeted Proteomics Assays.
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使用 CPTAC 检测门户来识别和实施高度特征化的靶向蛋白质组学检测。
DOI：
10.1007/978-1-4939-3524-6_13
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
Chen,Xian
中科院分区：
文献类型：
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作者：
Whiteaker,JeffreyR;Halusa,GoranN;Hoofnagle,AndrewN;Sharma,Vagisha;MacLean,Brendan;Yan,Ping;Wrobel,JohnA;Kennedy,Jacob;Mani,DR;Zimmerman,LisaJ;Meyer,MatthewR;Mesri,Mehdi;Boja,Emily;Carr,StevenA;Chan,DanielW;Chen,Xian
美国国家癌症研究所（NCI）的临床蛋白质组肿瘤分析联盟（CPTAC）推出了一个分析门户网站（ http://assays.cancer.gov ）作为一个开放源代码库的良好表征的靶向蛋白质组学测定。该门户旨在通过提供详细的分析性能表征数据、标准操作程序和试剂访问权限，来管理和传播高度表征的、基于靶向质谱（MS）的分析。检测内容可通过门户网站通过查询来访问，以查找针对与特定细胞途径、蛋白质复合物或特定染色体区域相关的蛋白质的检测。肽分析物的位置，其中有可用的测定是相对于其他功能的蛋白质，如序列结构域，异构体，单核苷酸多态性，翻译后修饰。总体目标是实现对所有人类蛋白质的稳健定量，并对基于MS的靶向测定的定量进行标准化，以最终实现随时间推移和跨实验室结果的协调。
The Clinical Proteomic Tumor Analysis Consortium (CPTAC) of the National Cancer Institute (NCI) has launched an Assay Portal ( http://assays.cancer.gov ) to serve as an open-source repository of well-characterized targeted proteomic assays. The portal is designed to curate and disseminate highly characterized, targeted mass spectrometry (MS)-based assays by providing detailed assay performance characterization data, standard operating procedures, and access to reagents. Assay content is accessed via the portal through queries to find assays targeting proteins associated with specific cellular pathways, protein complexes, or specific chromosomal regions. The position of the peptide analytes for which there are available assays are mapped relative to other features of interest in the protein, such as sequence domains, isoforms, single nucleotide polymorphisms, and posttranslational modifications. The overarching goals are to enable robust quantification of all human proteins and to standardize the quantification of targeted MS-based assays to ultimately enable harmonization of results over time and across laboratories.