Short communication: Genotyping and single nucleotide polymorphism analysis of bovine leukemia virus in Chinese dairy cattle
Short communication: Genotyping and single nucleotide polymorphism analysis of bovine leukemia virus in Chinese dairy cattle
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短讯：中国奶牛白血病病毒基因分型及单核苷酸多态性分析
DOI：
10.3168/jds.2018-15481
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发表时间：
2019
影响因子：
3.5
通讯作者：
Chengming Wang
中科院分区：
文献类型：
--
作者：
Yi Yang;S. Chu;Shaobin Shang;Zhangping Yang;Chengming Wang
牛白血病病毒（BLV）引起牛的地方性白血病，分为10种基因型，除几个欧洲国家、澳大利亚和新西兰外，分布于世界各地。尽管BLV在我国奶牛中广泛存在，个体水平的阳性率高达49.1%，但我国奶牛BLV基因型的研究却很少。为了解我国奶牛BLV的遗传变异性，本研究对我国5个城市的112份BLV阳性奶牛样本进行了BLV分子鉴定。采用邻接法对中国5个城市的BLV gp51部分env基因序列和GenBank中的53株BLV gp51基因序列进行系统发育分析，结果表明中国BLV gp51基因为基因6型。在中国毒株中总共鉴定出12个SNP，其中在盐城、上海和蚌埠毒株中鉴定出7个独特的SNP。BLV的基因分型结合SNP分析可作为一种有用的分子流行病学工具，用于追踪病原体的来源。这项研究强调了对地理上不同的BLV毒株进行遗传分析以了解BLV全球遗传多样性的重要性。
Bovine leukemia virus (BLV) causes enzootic leucosis in cattle and is classified into 10 genotypes with a worldwide distribution, except for several European countries, Australia, and New Zealand. Although BLV is widespread in Chinese cows with the positive rate of 49.1% at the individual level, very little is known about the BLV genotype in dairy cattle in China. To determine BLV genetic variability in cows in China, 112 BLV-positive samples from 5 cities in China were used for BLV molecular characterization in this study. Phylogenetic analysis using the neighbor-joining method on partial env sequence encoding gp51 obtained from 5 Chinese cities and those available in GenBank (n = 53, representing BLV genotype 1-10) revealed the Chinese strains belonged to genotype 6. Seven unique SNP were identified among Yancheng, Shanghai, and Bengbu strains out of the total 12 SNP identified in Chinese strains. The genotyping coupled with SNP analysis of BLV can serve as a useful molecular epidemiological tool for tracing the source of pathogens. This study highlights the importance of genetic analysis of geographically diverse BLV strains to understand BLV global genetic diversity.