Estimation of day of death using micro-segmental hair analysis based on drug use history: a case of lidocaine use as a marker
Estimation of day of death using micro-segmental hair analysis based on drug use history: a case of lidocaine use as a marker
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DOI：
10.1007/s00414-018-1939-9
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发表时间：
2019-01-01
影响因子：
2.1
通讯作者：
Inoue, Hiroyuki
中科院分区：
文献类型：
--
作者：
Kuwayama, Kenji;Nariai, Maika;Inoue, Hiroyuki
在非自然死亡的调查中，死亡时间通常是通过解剖检查来估计的。然而，很难准确确定死亡的日期，因为身体组织在死后的变化可能会受到身体所在位置的情况的很大影响。我们最近开发了一种估计药物摄入天数的方法，使用基于内部时间标记(ITMS)的微段毛发分析(ITMS)。在这种方法中，在收集毛发之前的特定时间间隔内摄取ITM，以标记个别发束内的时间尺度。以0.4毫米的间隔分割一根发束，对应于平均每天的毛发生长。最终通过计算含有药物的片段和发丝中的ITMS之间的距离来估计药物摄入的日期。在本研究中，该方法被应用于估计死亡日期。一具身体被发现，并在发现前57天记录了利多卡因给药手术的病史。用利多卡因作为ITM，对从身体上拔出的毛发进行了微段分析。在发丝的特定区域检测到利多卡因。使用已知的手术日期、发根到发束中利多卡因峰值的距离和平均毛发生长率来估计死亡日期。与传统的解剖学检查不同，使用头发的新估计方法使我们能够将估计的死亡时间范围缩小到死亡当天。基于吸毒史的微段毛发分析在确定非自然死亡时间方面非常有帮助。
During investigations of unnatural death, the time of death is generally estimated using anatomical examinations. However, it can be difficult to accurately determine the day of death, because postmortem changes in the body tissues can be greatly affected by the circumstances of the location of the corpse. We recently developed a method to estimate the day of drug ingestion, using micro-segmental hair analysis based on internal temporal markers (ITMs). In this method, ITMs are ingested at a specific time interval before hair collection to mark timescales within individual hair strands. A single hair strand is segmented at 0.4-mm intervals, corresponding to average daily hair growth. The day of drug ingestion is eventually estimated by calculating the distances between segments containing the drug and ITMs in a hair strand. In the present study, the method was applied to estimate the day of death. A corpse was discovered with a documented medical history of lidocaine administration for surgery 57days before the discovery. Micro-segmental analysis of a hair plucked from the corpse was performed using lidocaine as an ITM. Lidocaine was detected at specific regions in the hair strands. The day of death was estimated using the known surgery day, the distance from the hair root to the lidocaine peak in the hair strand, and the average hair growth rate. The novel estimation method using a hair enabled us to narrow the estimated time range of death up to the day of death, unlike the conventional anatomical examination. The micro-segmental hair analysis based on drug use history can be extremely helpful in determining the time of an unnatural death.