Zearalenone exposure affects epigenetic modifications of mouse eggs
Zearalenone exposure affects epigenetic modifications of mouse eggs
复制标题
玉米赤霉烯酮暴露会影响小鼠卵的表观遗传修饰。
DOI：
10.1093/mutage/geu033
复制
发表时间：
2014-11-01
期刊：
影响因子：
2.7
通讯作者：
Sun, Shao-Chen
中科院分区：
文献类型：
--
作者：
Zhu, Cheng-Cheng;Hou, Yan-Jun;Sun, Shao-Chen
玉米赤霉烯酮（ZEA）是一种由镰刀菌属真菌产生的真菌毒素，已被证明可引起家畜和人类的几例真菌中毒。然而，没有证据表明ZEA对小鼠卵发育能力的影响。在本研究中，我们发现ZEA处理对小鼠成熟卵母细胞的激活率有影响，表明ZEA影响卵的发育能力。并从表观遗传修饰的角度探讨了ZEA处理后小鼠成熟卵母细胞发育能力低下的可能机制。荧光强度分析显示，5-甲基胞嘧啶水平在ZEA处理后增加，表明处理后的鸡蛋中的总DNA甲基化水平增加。此外，组蛋白甲基化也发生了改变：在高剂量ZEA培养基中培养的卵子中，H3 K4 me 2以及H3 K9 me 3和H4 K20 me 1，me 2，me 3水平降低。因此，我们的研究结果表明，ZEA通过影响表观遗传修饰来降低卵的发育能力。
Zearalenone (ZEA) is a mycotoxin produced by various Fusarium fungi, which has been shown to cause several cases of mycotoxicosis in farm animals and humans. However, there is no evidence regarding the effect of ZEA on mouse egg developmental competence. In this study, we found that the activation rate of maturated oocytes was affected in mice by ZEA treatment, indicating that ZEA affects egg developmental competence. And we explored possible mechanisms of low mouse maturated oocyte developmental competence after ZEA treatment from an epigenetic modification perspective. The fluorescence intensity analysis showed that 5-methyl cytosine level increased after ZEA treatment, indicating that the general DNA methylation level increased in the treated eggs. Moreover, histone methylations were also altered: H3K4me2 as well as H3K9me3 and H4K20me1, me2, me3 levels decreased in eggs that were cultured in high-dose ZEA medium. Thus, our results indicated that ZEA decreased egg developmental competence by affecting the epigenetic modifications.