INCREASED VASCULAR ENDOTHELIAL GROWTH-FACTOR LEVELS IN THE VITREOUS OF EYES WITH PROLIFERATIVE DIABETIC-RETINOPATHY
INCREASED VASCULAR ENDOTHELIAL GROWTH-FACTOR LEVELS IN THE VITREOUS OF EYES WITH PROLIFERATIVE DIABETIC-RETINOPATHY
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DOI：
10.1016/s0002-9394(14)75794-0
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发表时间：
1994-10-01
影响因子：
4.2
通讯作者：
YEO, KT
中科院分区：
文献类型：
--
作者：
ADAMIS, AP;MILLER, JW;YEO, KT
测量并比较患有和不患有增殖性糖尿病视网膜病变的眼睛的血管生成多肽血管内皮生长因子（也称为血管通透性因子）的玻璃体水平。在玻璃体切除术时收集 20 只眼睛的未稀释玻璃体样本，并使用时间分辨免疫荧光测定法测定血管内皮生长因子水平。患有增殖性糖尿病视网膜病变的眼睛的玻璃体血管内皮生长因子水平显着高于没有增殖性糖尿病视网膜病变的眼睛（P = 0.006；Wilcoxon 秩和检验）。患有增殖性糖尿病视网膜病变的眼睛中的中位玻璃体浓度为29.1 pM，超过了体外血管内皮细胞最大增殖所需的已知浓度。这些数据与血管内皮生长因子在增殖性糖尿病视网膜病变中作为生理相关血管生成因子的作用一致。
The vitreous levels of the angiogenic polypeptide vascular endothelial growth factor (also known as vascular permeability factor) were measured and compared in eyes with and without proliferative diabetic retinopathy. Undiluted vitreous samples from 20 eyes were collected at the time of vitrectomy, and vascular endothelial growth factor levels were determined by using a time-resolved immunofluorometric assay. Vitreous vascular endothelial growth factor levels were significantly higher in eyes with proliferative diabetic retinopathy than in eyes without proliferative diabetic retinopathy (P = .006; Wilcoxon Rank Sum Test). The median vitreous concentration in the eyes with proliferative diabetic retinopathy was 29.1 pM and exceeded the known concentration required for the maximal proliferation of vascular endothelial cells in vitro. These data are consistent with vascular endothelial growth factor serving as a physiologically relevant angiogenic factor in proliferative diabetic retinopathy.