Gene mapping and candidate gene analysis of multi-grains 1 (Mg1) in rice
Gene mapping and candidate gene analysis of multi-grains 1 (Mg1) in rice
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水稻多粒1（Mg1）基因定位及候选基因分析
DOI：
10.1002/csc2.20100
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发表时间：
2020
期刊：
影响因子：
2.3
通讯作者：
Li Yunfeng
中科院分区：
文献类型：
--
作者：
Zhang Ting;You Jing;Zeng Xiaoqin;Yu Guoling;Zhang Yi;Li Yidan;Ye Li;Yao Wanyue;Tu Yujie;Ling Yinghua;Yao Hesheng;He Guanghua;Li Yunfeng
作为水稻的基本单位(Oryza sativaL.)花序，小穗对籽粒大小、重量和产量有深刻的影响。然而，小穗发育的分子机制一直没有完全阐明。在本研究中，我们鉴定了一个水稻小穗突变体多粒1号(Mg1)，该突变体是用甲烷磺酸乙酯处理‘西农1B’获得的。在g1突变体中，小穗发育出两个小花，具有额外的外膜状器官和更多的内部花器官。值得注意的是，小穗可以产生多粒。这些表型反映了利用mg1作为水稻增产手段的相当大的潜力。遗传分析表明，表型受单隐性基因控制。将MG1基因定位在6号染色体上RM20754和RM494之间的121.57 kb物理区域内。测序分析证实，在Os06g0717200(对应于花器官编号1[FON1])内，从G到A的单核苷酸替换导致了g1突变体中Gly-869到Asp-869的氨基酸突变。这一发现表明，MG1可能是FON1的一个新的等位基因。MG1通过调节与这些特性相关的基因的表达而参与小穗决定和花器官的发育。本研究结果详细描述了MG1的一个新功能，这可能有助于选育每穗粒数增加的水稻品种。
As the basic unit of rice (Oryza sativaL.) inflorescence, the spikelet has a profound influence on grain size, weight, and yield. However, the molecular mechanism that underlies the development of spikelets has never been fully elucidated. In this study, we characterized a rice spikelet mutant,multi‐grains 1(mg1), which was derived from treatment of ‘Xinong 1B’ with ethyl methanesulfonate. In themg1mutant, the spikelet developed two florets with an extra lemma‐like organ and an increased number of inner floral organs. Notably, the spikelet could produce multi‐grains. These phenotypes reflect the considerable potential of usingmg1as a means of increasing rice yield. Genetic analysis revealed that themg1phenotype is controlled by a single recessive gene. TheMG1gene was mapped to the interval between simple sequence repeat (SSR) markers RM20754 and RM494 on chromosome 6 within a physical region of 121.57 kb. Sequencing analysis identified a single‐nucleotide substitution from G to A withinOs06g0717200(which corresponds toFLORAL ORGAN NUMBER 1[FON1]), causing an amino acid mutation of Gly‐869 to Asp‐869 in themg1mutant. This finding suggests thatMG1may be a novel allele ofFON1.MG1is involved in the development of spikelet determinacy and floral organs by regulating the expression of genes associated with these characteristics. The present results detail a novel function ofMG1, which may facilitate the breeding of rice cultivars with increased numbers of grains per panicle.